机构地区: 华南农业大学理学院
出 处: 《西南大学学报(自然科学版)》 2009年第4期79-82,共4页
摘 要: 利用超滤膜分离装置对白术多糖进行超滤分离,按公子量分成5个级别,用苯酚-硫酸比色法分别测定不同分子量范围多糖的糖含量,结果表明:它们占总糖的比例分别为:≥100000的占3.39%,30000~100000的占10.47%,10000~30000的占5.42%,6000-10000的占20.04%,≤6000的占60.68%.另外各级多糖均具有抗氧化活性,其中10000~30000和≤6000分子量的白术多糖对超氧阴离子自由基(O2^-·)和羟基自由基(·OH)具有极强的消除作用. Atractylodes macrocephala polysaccharides were ultrafiltered by an ultrafiltration membrane separation unit, and they were divided into 5 levels in accordance with molecular weight (MW). The contents of polysaccharides for different MW were determined with the phenol-sulfuric acid method. The result showed that the proportion of the polysaccharides with different MW in the total polysaccharides were as follows: 3.39% for MW above 100 000, 10.47% for MW between 30 000 and 100 000, 5.42% for MW between 10 000 and 30 000, 20.04% for MW between 6 000 and 10 000, and 60. 68% for MW less than 6 000. Determination of antioxidant activity demonstrated that polysaceharides of different MW all had antioxidant activity, and polysaccharides with an MW between 10 000 and 30 000 and less than 6 000, in partitular, had a very strong seavenging action on O2^- · and · OH.