作　　者： ; ; ; ; ;

机构地区： 中国科学院亚热带农业生态研究所

出　　处： 《生态学报》 2009年第4期1763-1769,共7页

摘　　要： 基于土壤微生物遗传多样性随植被和季节的变化而变化的假设,运用变性梯度凝胶电泳技术(DGGE)检测岩溶区草丛(T)、灌丛(S)、次生林(SF)和原生林(PF)群落演替过程中土壤细菌和真菌群落的遗传多样性及其季节变化。随着地上植被的演替土壤细菌群落具有连续性但优势种群不明显,真菌群落没有连续性但优势种群明显。植被和季节对于细菌和真菌群落的Shannon多样性具有不同程度的显著影响,同时存在显著的植被和季节交互作用。草丛土壤中细菌和真菌群落的Shannon多样性有显著的季节变化(p<0.01);灌丛土壤中仅细菌群落多样性有显著季节变化(p<0.05);而森林土壤中细菌和真菌群落多样性没有显著的季节变化。土壤真菌和细菌多样性具有显著正相关关系。随着地上植被的正向演替,土壤微生物遗传结构逐渐稳定;植被恢复早期阶段,土壤中存在着丰富的微生物遗传多样性,但并不稳定。 The hypotheses that soil microbial taxonomic diversity responds with different vegetations and seasons,four successional communities：Tussock（T）,shrub（S）,secondary forest（SF）and primary forest（PF）,were tested in Huanjiang county,southwest China.The total soil microbial DNA was extracted,following the amplification of bacterial 16S and fungal 18S rDNA,and get fingerprinting by denaturing gradient gel electrophoresis（DGGE）in order to determine for these communities.In reverse with the soil bacterial communities,the soil fungal ones presented obviously dominant phylotypes but no continuity along successional ongoing.Significant effects of vegetation on soil microbial diversity measured by Shannon index（p〈0.05,p〈0.001,respectively）,but no prominent trends along successional process,were examined.Bacterial and fungal diversity also significantly affected by seasonal exchanges（p〈0.001,p〈0.001,respectively）,further more,these shifts（p〈0.01）of diversity in T soil other than in SF and PF soils were observed.Meanwhile,there exist some interaction effects between the vegetation and the season.Significant seasonal shifts（p〈0.01）in microbial taxonomic diversity in T soil,but not SF and PF soils were detected.Significant positive correlations were found between bacterial and fungal taxonomic diversities.Soil microbial taxonomic diversity gets steadier along vegetation succession and,in early restoration stage of degradation ecosystem in karst area,large but unstable microbial diversity exists in soils.

关 键 词： 微生物多样性 季节变化 植被效应

领　　域： [生物学] [生物学]