作　　者： ; ; ; ; ;

机构地区： 广西大学化学化工学院

出　　处： 《分析测试学报》 2009年第4期466-469,共4页

摘　　要： 建立了以金电极为工作电极电致化学发光测定盐酸克林霉素的方法,并采用循环伏安和电致化学发光法,研究了体系的电化学行为和电化学发光行为。研究结果表明,在0.1 mol/L的硼酸(pH8.0)缓冲溶液中,扫描速率为100 mV/s时,ECL的峰高与盐酸克林霉素浓度在1.0×10-5~1.0×10-4mol/L和1.0×10-7~8.0×10-6mol/L范围内呈线性关系,其线性回归方程分别为I(counts)=465.00×105c-133.80(r=0.996 8)和I(counts)=20.333×106c+100.25(r=0.995 9)。方法的检出限为1.0×10-7mol/L(S/N=3)。连续测定2.0×10-5mol/L的盐酸克林霉素溶液10次,发光强度值的RSD为1.74%。对样品进行加标回收率实验,回收率为93%~102%。该方法具有较高的选择性和灵敏度,样品处理简单快速,用于盐酸克林霉素胶囊的测定,结果满意。 Electrochemiluminescence（ECL） intensity of Ru（bpy）^2＋3 could be enhanced in the presence of clindamycin hydroehloride with the structure of tertiary amine in basic solution. Based on this, a novel ECL method was established for the determination of elindamycin hydrochloride at gold electrode. The electrochemical and ECL characteristics of Ru （bpy）^2＋3 - elindamycin hydrochloride system were investigated by ECL and cyclic voltammetrie （CV） method, and the experimental conditions were optimized. Under the optimal conditions, the enhanced ECL intensities had good linear relationship with the concentration of ctindamycin hydrochloride in the range of 1.0 × 10^-5 -1.0 × 10^-4 mol/L and 1.0 × 10^-7 -8.0 × 10^-6 mol/L, respectively, with a detection limit of 1.0 × 10^-7 moL/L. The RSD for 10 times determination ＇of 2.0 × 10^-5 moL/L clindamycin hydroehloride was 1.74% and the spiked recoveries were 93% - 102% . The method showed high selectivity and sensitivity and was applied in the determination of clindamyein hydrochloride in capsule with satisfactory results.

关 键 词： 联吡啶钌 电致化学发光 盐酸克林霉素 测定 金电极

领　　域： [理学] [理学]