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番石榴叶中多酚物质含量测定条件的优化
Optimization on Determination Conditions of Content of Phenol in Guava Leaf

作  者: ; ; ; (何洛强);

机构地区: 华南农业大学

出  处: 《农产品加工(下)》 2009年第3期181-183,192,共4页

摘  要: 研究了以没食子酸为标品测定番石榴叶多酚含量的适宜条件。结果表明,当福林试剂稀释10倍,使用质量分数为40%的Na2CO3,反应时间为7min,反应温度为50℃时,多酚含量在0~500mg内与吸光值呈良好的线性关系。测定方法的RSD为1.17%,平均回收率为98.4%,有较好的精密度和准确度。 Using gallic acid as the standard, the optimized conditions were determined for the content of guava leaf phenol. The results showed that when the dilution times were 10-fold, the content of Na2CO3 was 40%, reaction temperature was 50 ℃, and reaction time was 7 rain, a good linearity between content of phenol and absorbance was obtained in the range of 0-500 mg. The RSD of determination was 1.17%, average recovery rate of spiked phenol was 98.4%, which had good precision and accuracy.

关 键 词: 番石榴叶 多酚 福林法

领  域: [化学工程]

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