作　　者： ; ; ;

机构地区： 广西科学院

出　　处： 《现代化工》 2010年第S2期128-131,133,共5页

摘　　要： 将实验室构建的产粘质沙雷氏菌脂肪酶的基因工程菌于20 L发酵罐中发酵,并将分离提取获得的粗脂肪酶液用sol-gel包埋法和大孔吸附树脂吸附两种方法进行固定化,考察了醇油比、含水量、反应温度、反应时间、催化剂用量对生物柴油产率的影响。实验结果显示采用大孔树脂吸附法制备的固定化酶的催化效果优于sol-gel包埋法制备的固定化酶,在优化条件下,以大孔树脂吸附法制备的固定化酶可使生物柴油的单批转化率达到88%。 High-yield gene engineering E.coli strain which is transformed in serratia marcescens lipase is incubated at 20 L fermenter,the lipase is extracted and immoblized by two ways.The first is immobilized within a chemically inert,hydrophobic sol-gel support which prepared by polycondensation of hydrolyzed tetramethoxysilane and methyltreimethoxysila.The second is physical adsorption onto macroporous reins HPD100.Using these immobilized lipase,the effects of water and methanol concentration,temperature,reaction time in the transesterification reaction are investigated.The experiment result reveal that the macroporous reins immobilized lipase is better than sol-gel immobilized lipase.With macroporous rein immobilized lipase,the batch conversion rate can reach 88%.

关 键 词： 粘质沙雷氏菌 脂肪酶 基因工程菌 包埋法 生物柴油 固定化酶

领　　域： [生物学]