作　　者： ; ; ; (任献忠）;

机构地区： 华中农业大学食品科学技术学院

出　　处： 《中国食品学报》 2009年第1期59-65,共7页

摘　　要： 为开发杨梅花色苷资源,对杨梅花色苷的提取分离工艺进行了研究。通过对乙醇浓度、提取温度、pH值、提取时间和料液比等单因素的浸提效果的比较分析,确定了3个主要的影响因子,即:提取温度、料液比、乙醇浓度。通过正交试验得到杨梅花色苷的最佳提取工艺条件,即:乙醇体积分数70%、提取温度40℃、提取液pH3、提取时间2h、料液比1∶10。在此条件下,杨梅花色苷的提取率为91.83%。利用AB-8、D101和ADS-173种大孔吸附树脂分离杨梅花青素,结果表明:ADS-17对杨梅花色苷基本无吸附,AB-8和D101分离杨梅花色苷的效果相近。以体积分数60%的乙醇溶液为洗脱剂,上样pH3时,两种大孔树脂分离得到的提取物中花色苷得率分别为70.00%和67.08%,产品得率分别为1.44%和1.38%。 In order to develop anthocyanin source in Myrica rubra, extraction and purification technology of anthocyanins from Myrica rubra were studied. The best level of each factors including ethanol concentration, extraction temperature, pH value, extraction time and the ratio of sample to solvent （W/V） in extraction of anthocyanin from Myrica rubra was determined. Orthogonal experiment of three major influence factors： extraction temperature, the ratio of sample to solvent（W/V） and ethanol concentration were proceed. The optimum extraction technology are as follows： ethanol concentration 70%, extraction temperature 40 ℃, pH3, extraction time 2 h, the ratio of sample to solvent volume （W/V） 1： 10. Under this condition, the extraction rate of anthocyanin was 91.83%. The anthocyanin was further purified with AB-8, D101 and ADS-17 resin, the results showed that the separation effects of AB-8 and D101 on anthoeyanin from Myrica rubra were better with 60% ethanol （pH3） as eluate. The yield rates of anthocyannin were 70.00% and 67.08% respectively, and the product rates were 1.44% and 1.38%.

关 键 词： 杨梅 花色苷 提取 纯化

领　　域： [轻工技术与工程] [轻工技术与工程] [生物学]