作　　者： ; ; ; ; ;

机构地区： 华南农业大学

出　　处： 《植物病理学报》 2009年第1期100-103,共4页

摘　　要： ORFⅡ gene of Banana streak virus GuangDong isolate(BSV-GD) was amplified from a BSV-GD recombinant plasmid by PCR,and the gene was expressed by being cloned into prokaryote expression vector pET-28b(+).The fusion protein was about 16.5 kDa in size and was soluble with SDS-PAGE analysis.The purified protein was obtained by using the histidine labeling kit of N-terminus of protein.The antiserum was obtained by immunizing healthy rabbits with the purified protein.Western blot and ELISA analysis showed that the special antiserum of BSV possessed high titer,which was tested as 1∶51 200.The study was a base for further research on BSV including ORFⅡ gene function and virus detection. ORFⅡ gene of Banana streak virus GuangDong isolate （BSV-GD） was amplified from a BSVGD recombinant plasmid by PCR, and the gene was expressed by being cloned into prokaryote expression vector pET-28b（ ＋ ）. The fusion protein was about 16.5 kDa in size and was soluble with SDS-PAGE analysis. The purified protein was obtained by using the histidine labeling kit of N-terminus of protein. The antiserum was obtained by immunizing healthy rabbits with the purified protein. Western blot and ELISA analysis showed that the special antiserum of BSV possessed high titer, which was tested as 1：51 200. The study was a base for further research on BSV including ORF Ⅱ gene function and virus detection.

关 键 词： 香蕉生产 病毒病 抗体制备 原核表达 线条 基因 植株矮化

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]