作　　者： ; ; ;

机构地区： 韶关学院英东农业科学与工程学院

出　　处： 《安徽农业科学》 2009年第7期3225-3227,共3页

摘　　要： [目的]寻找合适的苦瓜多糖脱蛋白方法。[方法]比较了Seveage法、酶法、酶-Seveage结合法、三氯乙酸法以及盐酸法对苦瓜粗多糖的脱蛋白效果。[结果]酶-Seveage结合法的脱蛋白效果最佳,木瓜蛋白酶用量为1.25%(V/V),pH 6.5,温度60℃,时间为2 h,再用Se-veage试剂脱蛋白1次,蛋白质去除率达77.2%,多糖损失率仅为19.3%。[结论]酶与Seveage结合法是一种有效的脱蛋白方法,此法可以较好地脱除游离蛋白,并使多糖损失较少。 [Objective] The research aimedt to seek the suitable deproteinization methods from Mornordica charantia L.polysaecharides. [Method] The deproteinization effects of Seveage method, enzyme method, enzyme-Seveage method, trichloroacetie acid method and hydrochloric acid method on M. charantia polysaccharides were compared. [Result] The deproteinization effect of enzyme-Seveage method was the best,with the papain dose of 1.25%（V/V）, pH 6.5 at 60℃ for 2 h.Then the polysaecharide solution was deproteinized again with Seveage agent and the removal rate of protein reacbed 77.2% and the loss rate of polysaccharide was 19.3 %. [ Conclusion] The enzyme-Seveage method was an effective deproteinization method, with higher removal of dissociated protein and lower loss of polysaccharide. Key words

关 键 词： 苦瓜 多糖 脱蛋白

领　　域： [生物学] [理学] [理学]