作　　者： ; ; ; ; ;

机构地区： 华南农业大学资源环境学院农药与化学生物学教育部重点实验室

出　　处： 《昆虫学报》 2009年第2期147-152,共6页

摘　　要： 研究了腈菌唑导致的细胞膜穿孔对离体培养斜纹夜蛾Spodoptera litura卵巢细胞（SL细胞）膜通透性的影响。结果表明：腈菌唑可使不能穿过SL细胞膜的大分子物质荧光染料PI大量穿过细胞膜进入胞内，20和40μg／mL腈菌唑处理后12h，SL细胞荧光强度增加率分别为11．95％和25．80％；处理后24h，SL细胞荧光强度增加率分别为27．77％和57．49％。腈菌唑也可明显提高SL细胞内大分子物质乳酸脱氢酶的漏出率，20和40μg／mL腈菌唑处理sL细胞24h后，胞内乳酸脱氢酶漏出率分别为30．66％和43．93％；处理后48h，漏出率分别为41．22％和57．91％。腈菌唑可以明显提高SL细胞胞内钙离子含量，20和40μg／mL腈菌唑处理sL细胞24和48h，胞内钙荧光强度与对照差异显著。处理后24h，腈菌唑对SL细胞的LC50值为35．84μg／mL，明显高于典型细胞毒剂鱼藤酮的活性。当质量比为1：1和2：1时，腈菌唑与鱼藤酮联用对sL细胞处理后24h的LC50值为43．92和26．09μg/mL，共毒系数分别为137．60和188．49，增效作用显著，随着腈菌唑的含量增加，增效作用增加。腈菌唑对SL细胞具有良好的抑制作用，可以打通限制物质穿透的细胞膜屏障，提高农药活性成分的有效利用率。 The effect of cell membrane perforation caused by myclobutanil on the cell membrane permeability of Spodoptera litura ovary cells （SL cells） that were cultured in vitro was assayed in this study. The results showed that a large number of fluorescent dye PI, which was macromolecular material and could not pass through the SL cell membrane, passed through the cell membrane and entered into cells with the myclobutanil. The increasing rates of cell fluorescence intensity were 25.80% and 11.95% respectively after SL cells were treated with 20 and 40 μg/mL myclobutanil for 12 h, and 27.77% and 57.49% respectively for 24 h. Myclobutanil significantly improved the leakage rate of lactate dehydrogenase which is the macromolecular material in SL cells. The leakage rates were 30.66% and 43.93% respectively after SL cells were treated with 20 and 40 μg/mL myclobutanil for 24 h, and 41.22% and 57.91% respectively for 48 h. Myclobutanil significantly increased the intracellular calcium ion content. Intracellular calcium fluorescence intensity was significantly different from control after SL cells were treated with 20 and 40 μg/mL myelobutanil for 24 and 48 h. After SL cells were treated with myclobutanil for 24 h, the LC50 was 35.84μg/mL. The activity of myclobutanil was significantly higher than that of rotenone which was a typical cell toxicant. When the SL cells were treated with the mixture of myclobutanil and rotenone at the mass ratios of 1 ： 1 and 2：1 for 24 h, the LC50 values were 43.92 and 26.09μg/mL respectively, and the co-toxicity coefficients were 137.60 and 188.49 respectively. The results showed that the synergism of myelobutanil and rotenone is remarkable and increased with the myclobutanil content increasing. These results suggest that myelobutanil has a good inhibition on the SL cells, can get through the membrane barrier that limits materials to penetrate the cells, and so increases the effective utilization of pesticide active ingredients.

关 键 词： 斜纹夜蛾 腈菌唑 膜穿孔 卵巢细胞 通透性 联合作用

分 类 号： [Q965.9]

领　　域： [生物学]