作　　者： ; ;

机构地区： 南京农业大学动物医学院

出　　处： 《南京农业大学学报》 1998年第1期91-95,共5页

摘　　要： ３６头杂交山羊随机分成３组，第Ⅰ组静脉注射生理盐水作为对照；第Ⅱ组静脉注射大肠杆菌（Ｏ１１１Ｂ４）内毒素，１８００Ｕ／ｋｇ，注射两次，间隔２４ｈ；第Ⅲ组处理同第Ⅱ组，但于第２次注射内毒素前１０ｍｉｎ静脉注射山莨菪碱２．５ｍｇ／ｋｇ。分别于试验后第５和１２ｈ各组剖杀６头，检测肾组织中丙二醛（ＭＤＡ）含量和抗氧化参数。结果表明：第Ⅱ组肾组织ＭＤＡ含量明显升高（Ｐ＜０．０１），谷胱甘肽过氧化物酶（ＧＳＨ－Ｐｘ）、谷胱甘肽还原酶（ＧＲ）、总超氧化物歧化酶（Ｔ－ＳＯＤ）、锰－ＳＯＤ、铜锌－ＳＯＤ活性均显著下降（Ｐ＜０．０５），并伴有还原型谷胱甘肽（ＧＳＨ）、总巯基（Ｔ－ＳＨ）、非蛋白巯基（ＮＰ－ＳＨ）、蛋白结合巯基（ＰＢ－ＳＨ）含量明显降低（Ｐ＜０．０１，Ｐ＜０．０５）；第Ⅲ组肾组织ＭＤＡ含量在试验后５ｈ低于Ⅱ组（Ｐ＜０．０５），试验后１２ｈ与Ⅰ组差异不显著（Ｐ＞０．０５），ＧＳＨ－Ｐｘ、ＧＲ、ＣｕＺｎ－ＳＯＤ和试验后５ｈ的Ｔ－ＳＯＤ、Ｍｎ－ＳＯＤ活性均高于Ⅱ组（Ｐ＜０．０５），ＧＳＨ、Ｔ－ＳＨ、ＮＰ－ＳＨ、ＰＢ－ＳＨ含量亦高于Ⅱ组（Ｐ＜０．０５），其中Ｔ－ＳＨ、ＰＢ－ＳＨ与Ⅰ组无明显差异。提示在山羊内毒素休克时，肾? Thirty six hybrid goats were randomly divided into three groups. Group Ⅰ was the normal control. The goats of Group Ⅱ were intravenously injected E. coli (O 111 B 4) endotoxin with dose of 1 800 endotoxin unit per kilogram body weight for 24 h apart. The goats of Group Ⅲ were treated with the same as Group Ⅱ and intravenously given 2.5 mg anisodamine per kilogram body weight for 10 minutes before the second endotoxin administration. Six goats of every group were killed 5 h and 12 h after endotoxin injection. Renal tissue MDA contents and antioxidant parameters were examined respectively. The results showed as follows. The renal tissue MDA contents of Group Ⅱ significantly increased ( P <0.01). The activities of renal GSH Px, GR, T SOD, Mn SOD and CuZn SOD markedly decreased followed with reduction of the levels of GSH, T SH, NP SH and PB SH of renal tissues in Group Ⅱ. However, the renal tissue MDA contents of Group Ⅲ were lower than those of Group Ⅱ at 5 h and not different with Group Ⅰ at 12 h. The activities of GSH Px, GR, CuZn SOD and T SOD, Mn SOD of Group Ⅲ at 5 h were higher than those of Group Ⅱ ( P <0.05). The contents of renal tissue GSH, T SH, NP SH and PB SH of Group Ⅲ were more than those of Group Ⅱ. There were no difference of renal tissue T SH and PB SH between Group Ⅰ and Ⅲ. The experimental evidence indicated that renal antioxidant function significantly decreased and renal lipid peroxidation injury induced by free radical during endotoxic shock of goats, but anisodamine given before endotoxin administration could markedly reduce the renal tissue injury.

关 键 词： 山羊 内毒素休克 肾脏 自由基 山莨菪碱

领　　域： [农业科学] [农业科学] [农业科学]