机构地区: 海南大学环境与植物保护学院
出 处: 《中国生物防治》 2009年第1期60-65,共6页
摘 要: 在香蕉枯萎病重病园区,从生长正常香蕉假茎内分离获得一株细菌E353菌株。经对峙培养、孢子萌发抑制测定,E353对香蕉枯萎病菌菌丝生长、孢子萌发具有良好抑制效果。盆栽试验表明,E353活菌培养液(750ml/株)浸根处理,对香蕉枯萎病的防效为60.67%。经形态学、生理生化和16S rDNA序列比对,将E353鉴定为菊欧氏杆菌Erwinia chrysanthemi。 An endophytic strain E353 was obtained from the pseudostem of healthy banana plant in a field heavily infected with Fusarium oxysporum f. sp. cubense (FOC). Antagonism of the strain against FOC was tested via dual-culture, inhibition test on conidia germination, and pot trials. Results showed that E353 effectively inhibited mycelium growth and conidia germination. Efficacy of strain E353 to control the wilt disease was 60.67 % in pot trials. Strain E353 was identified as Erwinia chrysanthemi according to its characteristics in morphology, physiology, biochemistry and 16S rDNA sequence.