作　　者： ; ; ; ; ;

机构地区： 广东海洋大学水产学院

出　　处： 《海洋科学》 2009年第2期55-59,共5页

摘　　要： 用添加不同剂量L-精氨酸(L-arg)的饲料投喂凡纳滨对虾(Litopenaeus vannamei),测定其血淋巴液中一氧化氮(NO)含量以及一氧化氮合成酶(NOS)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)和超氧化物歧化酶(SOD)活力,并检测其对病原体的抗病力。结果显示:L-arg显著提高血淋巴液中NO含量,且变化趋势与NOS、AKP活性升高趋势相一致;中等剂量(质量分数为1%)L-arg显著提高血淋巴液中SOD和ACP活性,但高剂量(质量分数为1.5%)、低剂量(质量分数为0.5%)L-arg对血淋巴液中SOD(高剂量除外)和ACP活性影响不显著。副溶血弧菌人工感染后,中剂量L-arg组(1%)对虾死亡率最低,高剂量(1.5%)、低剂量组(0.5%)次之,对照组死亡率最高,各剂量L-arg组死亡率与对照组相比差异显著。表明饲料中添加适量L-精氨酸可使对虾体液免疫因子总体活力达到较高水平,L-精氨酸含量过高对对虾非特异免疫力的作用效果反而不佳。 The American white shrimp Litopenaeus vannarn of the body length 6.8 - 8.5 cm was collected from a culture farm in Zhanjiang. One control group （raw diets） and three levels of L-arginine （L-arg） groups were designed. The levels of L-arginine added in the raw diets were 1.5 %, 1% and 0.5 %, respectively. The period was 4 weeks. Humoral immuning factors such as NOS, NO, ACP, AKP and SOD, as well as the sensitivity of the shrimp to Vibrio parahaemolyticus were investigated. The results showed that L-arginine at doses from 0.5 % to 1.5 % induced the increase of serum NO levels, which were in accordance with the increase of serum NOS and AKP levels. Both serum SOD and ACP levels increased significantly in the 1% L-arg -fed group, however, there was no significant difference in SOD（ except for SOD in 1. 5% L-arg -fedgroup） and ACP levels in 1.5 % and 0.5 % L-arg -fed groups. The lowest death rate was found in 1% L-arg-fed group, then in the 0.5% and 1.5% L-arginine-fed groups, and the highest death rate was found in the control group under infection V. parahaernolyticus. There was a significant difference in death rate between the control group and the L-arg-fed groups. The results suggested an adequate added level of L-arginine （e. g. 1%） could increase the activities of the immune factors in shrimp up to a high level, but the higher added level would not have a good effect on the non-specific immunity in shrimp.

关 键 词： 凡纳滨对虾 精氨酸 一氧化氮合成酶 酸性磷酸酶 碱性磷酸酶 超氧化物歧化酶

领　　域： [农业科学] [农业科学]