作　　者： ; ; ; ;

机构地区： 华南农业大学食品学院

出　　处： 《食品与发酵工业》 2008年第12期176-179,共4页

摘　　要： 以香蕉为原料,采用水浸提得到粗糖液,通过SephadexG-25柱层析纯化得到香蕉低聚糖,并初步分析所得低聚糖的理化性质。结果表明:水浸提香蕉低聚糖的优化工艺条件是:料液比为(g:mL)1∶2,温度为60℃,提取时间为60min,提取2次;凝胶柱层析操作条件为:进样体积1.0mL,洗脱液流速为10s/滴,收集体积2.0mL,得到2种低聚糖;香蕉低聚糖-1为浅黄色粉末,无臭,水溶性不好,吸湿性不强;香蕉低聚糖-2为黄色粉末,无臭,水溶性良好,有一定的吸湿性;凝胶渗透色谱法测出香蕉低聚糖-2的数均分子质量为1313。 Banana oligosaccharides,obtained from bananas by water extraction and ethanol precipitation, were separated and purified by using the pillar SephadexG-25. The optimized processing of banana oligosac- charide extraction was ： ratio of material and water （ρm：v） 1 ： 2, 60℃, extracting 60min and extracting twice. The column chromatography operation was 2.0mL/tube injection volume, distilled water as the carrier solution at a flow rate of 10s/drop, 2.0mL/tube collection volume. Two kinds of of oligosaccharides dehydrate powder by freeze--drying were obtained. Banana oligosaccharide--1 was light yellow powder,odorless, with lower water--solubility and moisture absorption. Banana oligosaccharide--2 is yellow powder, odorless,with higher water--solubility and moisture absorption. The molecular weight of banana oligosac- charide--2 analyzed by gel permeation chromatography was 1 313.

关 键 词： 香蕉 低聚糖 提取 柱层析 性质

领　　域： [轻工技术与工程] [轻工技术与工程] [理学] [理学]