作　　者： ; ; ; ; ;

机构地区： 山东农业大学园艺科学与工程学院

出　　处： 《华南农业大学学报》 2009年第1期110-112,共3页

摘　　要： 从广东和山东呈现花叶病症的辣椒上分离纯化得到2个黄瓜花叶病毒Cucumber mosaic virus(CMV)的分离物.利用RT-PCR技术克隆了病毒分离物的外壳蛋白(CP)基因.核苷酸序列测定表明,CMV-GDLJ和CMV-SDLJ 2个病毒分离物CP基因序列长度为657bp,编码218个氨基酸.2个病毒分离物的CP基因序列与CMV亚组Ⅰ的6个株系CP基因序列同源性均在90%以上,而与亚组Ⅱ的3个株系同源性均低于80%,据此将分离物CMV-GD和CMV-TA归属于CMV亚组Ⅰ. Two CMV isolates were obtained from the pepper with mosaic symptom in Guangdong and Shandong. The coat protein （CP） gene of the isolates was cloned by using RT-PCR technology. Sequencing results showed that the CP gene of the isolate had 657bp, encoding 218 amino acids. Comparison of the CP gene nucleotide sequences indicated that the CP gene nucleotide sequences of the isolate shared over 90% of homology with that of 6 strains belonging to CMV subgroup I , but less than 80% with 3 strains of subgroup Ⅱ. Based on the homology, it was concluded that the two isolates belong to subgroup I.

关 键 词： 辣椒 黄瓜花叶病毒 亚组 基因 序列分析

领　　域： [农业科学] [农业科学]