作　　者： ; ; ; ; ;

机构地区： 华南农业大学兽医学院农业部养禽与禽病防治重点开放实验室

出　　处： 《华南农业大学学报》 2009年第1期74-80,共7页

摘　　要： 对我国鸭病毒性肝炎病毒(Duck hepatitis virus,DHV)分离株YZ和CL的全基因组进行了序列测定分析.结果表明,2分离株的基因组全长分别为7 713 nt和7 709 nt,包括626 nt的5′UTR、6 747 nt的ORF、314 nt的3′UTR以及23 nt和19 nt的poly(A)尾.结构蛋白VP0含有保守的四肽CPRP,VP3的N端存在一段约20个氨基酸的碱性氨基酸富集区域;VP1缺少RGD(Arg-Gly-Asp)基序.DHV2分离株存在3个不相关的2A蛋白基序,其中2A1蛋白类似口蹄疫病毒属的2A蛋白,2A2蛋白与AIG1蛋白(拟南芥)和GTPase更为相似,2A3蛋白则与副肠孤病毒属的2A2蛋白类型相似;2C蛋白含有保守基序EPxxxxxxGxxGxGK(S/T)、QxxxxxDDxxQ和KGxxxxSxxxxxS/T.3C蛋白含有催化三联体H38-D69-C144;3D蛋白包含KDELR、DxxxxD、GxxSGxxxTxxxNx、YGDD和FLKR等RNA聚合酶的特征基序.基于多聚蛋白、VP1、2C和3C蛋白的进化关系分析结果均表明,DHV分离株与副肠孤病毒属的遗传关系最近,但占据一个清晰的独立遗传谱系,应将DHV单独划属. The genome sequences of duck hepatitis virus type 1 （ DHV-1 ） strains YZ and CL isolated from China were determined and analysed. The length of the genomes were 7 709 or 7 713 nt and contained 626 nt of 5＇untranslated region（ UTR）, a single, large open reading frame of 6 747 nt,314 nt of 3＇ untranslated region. Analysis of the amino acid sequences of DHV with other picornavirus indicated that conserved motif CPRP existed in DHV capsid polypeptide VP0, a 20 aa region with rich basic amino acid in VP3 protein, and the Arg-Gly-Asp（RGD） motif was not present in VP1 protein. The 2A1 protein of DHV-1 was an aph- thovirus-like 2A protein;the 2A2 protein contained a putative conserved AIG1 domains and exhibited a significant similarity to GTPase of immunity associated protein（GIMAP） family ;the 2A3 protein was similar to the 2A2 protein of Parechovirus. The 2C sequence EPxxxxxxGxxGxGK（S/T）, QxxxxxDDxxQ and KGxxxx- SxxxxxS/T, which conform with the conserved nucleic acid binding domain of the putative picornavirus heliease. The 3C^pro contained a catalytic triad formed by histidine, aspartate and cysteine,at position 38,69, 144 respectively. Predicted domains of eight RNA-dependent RNA polymerase motifs were aligned in 3D RNA dependent RNA polymerase region, motifs KDELR, DxxxxD, GxxSGxxxTxxxNx, YGDD and FLKR of were highly conserved. Phytogenefic and evolutionary analysis showed that DHV-1 belongs to a new group of the family Picornaviridae that may form a separate genus most closely related to the genus Parechovirus.

关 键 词： 鸭病毒性肝炎病毒 分离株 基因组测序 基因组分析

领　　域： [农业科学] [农业科学] [农业科学]