作　　者： ; ; ; ;

机构地区： 深圳大学生命科学学院过敏反应与免疫学研究所

出　　处： 《中国乳品工业》 2008年第12期15-17,共3页

摘　　要： 对牛奶过敏原进行分离、鉴定与纯化。通过SDS-PAGE电泳分离牛奶的蛋白质组份,采用免疫印迹(Western-blotting)方法鉴定过敏原,通过离子交换层析对牛奶过敏原进行初步纯化。结果表明,鲜牛奶粗提液SDS-PAGE显示蛋白条带有12条,奶粉粗提液SDS-PAGE显示出的蛋白条带与鲜牛奶的蛋白条带基本一致。鲜牛奶Western-Blotting显示14ku的阳性条带。离子交换层析可初步纯化出14ku的过敏原蛋白。本研究对牛奶过敏原进行了分离和鉴定,并初步纯化出牛奶的主要过敏原。 To isolate, identify and purify the allergens in milk. The total protein of the purple snapper was isolated by SDS-PAGE. The allergens of purple snapper were identified by Western-blotting. To purify the allergens, ion exchange chromatography was employed. There were 12 protein bands visible on the SDS-PAGE gel in fresh milk and milk powder. The allergens whose molecular mass was 14 ku were identified by Western-blotting. The 14 ku of milk was purified by ion exchange chromatography. The allergens of milk were isolated and identified. And the allergens of milkr were purified primarily.

关 键 词： 牛奶 过敏原 免疫印迹 离子交换层析

领　　域： [轻工技术与工程] [轻工技术与工程]