作　　者： ; ; ; ; ;

机构地区： 西北农林科技大学动物科技学院

出　　处： 《中国兽医学报》 2008年第12期1426-1429,共4页

摘　　要： 分离妊娠山羊子宫内膜淋巴细胞（Endometrial lymphocyte，EML）后，分别加入4、8、12、16mg／L的亚硒酸钠进行体外培养，探讨硒对EML的活化作用及其对分泌细胞因子IL-2、IL-4、TGFβ1及TGFβ2的影响。结果显示，8～16mg／L的亚硒酸钠可显著地促进山羊EML的体外活化（P〈0．01），并呈剂量依赖性。EML活化后，对IL-2的分泌呈微弱的促进作用；对IL-4、TGFβ1、TGFβ2的分泌有显著的促进作用。 This paper was designed to test the in vitro activation of endometrial lymphocytes（EML）by Se. EML was isolated from pregnant goat,in vitro cultured in media respectively containing 4,8,12,16 mg/L sodium selenite and their activation in vitro and secretion of IL-2,IL-4,TGF-β1 and TGF-β2 were assayed. The results showed that 8 16 mg/L sodium selenite significantly activated goat EML in a dose-dependent manner （P〈0.01）, and 4 mg/L sodium selenite also activated the goat EML but not significantly to control groups （P〉0.05）. The activated EML with sodium selenite in vitro slightly promoted the secretion of IL-4 by goat EML, and significantly （P〈0.01） stimulated the secretion of IL-4,TGF-β1 and TGF-β2 by EML.

关 键 词： 硒 山羊 子宫内膜淋巴细胞 细胞因子

领　　域： [农业科学] [农业科学] [农业科学] [农业科学] [农业科学] [农业科学]