作　　者： ; ; ; ; ;

机构地区： 中国科学院亚热带农业生态研究所

出　　处： 《环境科学学报》 2008年第12期2590-2596,共7页

摘　　要： 基于土壤细菌代谢多样性和地上植被多样性具有协同关系的假设,利用BIOLOG技术研究西南典型喀斯特地区植被演替的不同阶段(草丛(T)、灌丛(S)、次生林(SF)和原生林(PF))土壤中细菌代谢多样性的分异特征.结果表明,植物群落和土壤细菌代谢Shannon’s多样性指数分别遵循如下变化趋势:SF>PF>S>T, SF/PF>S>T.双因素方差分析结果显示,植被和植被与季节的交互效应对土壤细菌代谢Shannon’s多样性有显著性影响(分别为p<0.001,p<0.05),季节变化没有显著影响(p>0.05);但同一植被下,独立样本t检验表明,细菌功能多样性仅在PF土壤中有显著的季节变化(p<0.01),而在其余土壤中季节变化不显著(p>0.05).多元方差和主成分分析表明,4种植物群落的土壤细菌利用碳源的模式具有显著差异(p<0.001);土壤细菌群落利用碳源的模式聚为3类:T、S (夏季)、SF(PF)和S (冬季).因此,植被的严重退化会降低土壤的微生物代谢多样性,而适度的人为干扰森林群落不会导致土壤细菌代谢功能的降低;灌丛土壤环境是植被演替过程中的过渡阶段,其孕育的土壤细菌具有森林阶段土壤细菌的部分特征. On the basis of the hypothesis that a positive correlation exists between plant diversity and soil microbial metabolic diversity, the metabolic diversity characteristics of a soil microbial community were compared using BIOLOG_Eco microplates in different successional communities （tussock （T） , shrub（S） , secondary forest （SF） and primary forest （PF）） from Huanjiang County, southwest China. The values of Shannon＇s diversity in the plant community were in the order： SF 〉 PF 〉 S 〉 T, and soil metabolic function from highest to lowest followed the order SF/PF 〉 S 〉 T. Two-way ANOVA showed that both vegetation （p 〈 0. 001 ） and the interactions between vegetation and season （p 〈 0. 05 ） had significant effects on Shannon＇s diversity of soil microbial metabolic diversity, but the seasonal effect itself was insignificant （p 〉 0.05 ）. However, for the same vegetation type, independent t-test showed thai seasonal changes of microbial metabolic diversities in PF soils were significant （p 〈 0.01 ） , but other types of community showed unchanged patterns. The results of MANOVA and PCA indicated that there were highly significant differences （p 〈0. 001 ） in community-level physiological profiling patterns （CLPPs） among the four soil microbial communities, and CLPPs were clustered in three pans： T, S （June） and SF/PF/S （Dec）. Therefore, the serious degradation of vegetation would decrease the soil microbial metabolic diversity, but the diversity of natural forest would not be affected by modest anthropogenic disturbance. The S soil environment, with partly similar bacterial metabolic characteristics in forest soils （SF and PF） , might be a transitional stage during the process of vegetation succession.

关 键 词： 细菌群落 季节动态 植被效应

领　　域： [环境科学与工程]