作　　者： ; ; ; ; ;

机构地区： 山东农业大学农学院

出　　处： 《生态学报》 2008年第11期5370-5376,共7页

摘　　要： 以山东泰安、临沂、莱芜、菏泽和潍坊5个居群的72份丹参株系为材料,利用ISSR引物进行群体遗传结构的研究。结果表明:8个ISSR引物在5个居群中共扩增出219个位点,平均可扩增出27条带,在种级水平及泰安、临沂、莱芜、菏泽和潍坊5个居群水平多态性位点百分比分别为98.63%、81.28%、66.67%、66.21%、51.14%和50.68%,种级水平的Nei基因多样性和Shannon信息指数大于各居群;5个居群Nei基因多样性和Shannon信息指数相比较,泰安>临沂>莱芜>菏泽>潍坊;根据基因分化系数,测得的基因流值Nm为4.2352;UPGMA聚类分析结果表明,莱芜居群和临沂居群遗传一致度最大,遗传关系最近,泰安居群与其它4个居群遗传关系最远;分析发现菏泽居群、泰安居群是相对独立的群体,但5个居群间存在部分基因交流。所有参数分析表明,泰安居群遗传多样性最丰富,故在制定原位种质保护计划时应优先考虑泰山周边地区的丹参。 72 Salvia miltiorrhiza lines from five different geographical populations located at Tai＇an, Linyi, Laiwu, Heze and Weifang in Shandong province of China were studied by ISSR markers. The results indicated that 219 polymorphic bands were detected with 8 primers, average twenty-seven bands every primer. The percentage of polymorphic loci （P） was 98.63% , 81.28% , 66.67%, 66.21% , 51.14% and 50.68% , respectively at species level and Tai＇an, Linyi, Laiwu, Heze and Weifang population level. The Nei＇s gene diversity index and Shannon＇s information index of species level were higher than population level. The Nei＇s gene diversity index and Shannon＇ s information index of five populations were Tai＇ an 〉 Linyi 〉 Laiwu 〉 Heze 〉 Weifang. Gene flow between the populations was 4. 2352 based on genetic differentiation coefficient. UPGMA cluster analysis indicated that Laiwu population and Linyi population were the highest in Genetic identity which was the nearest in genetic relationships, and the genetic distance between Tai＇an population and other four populations were the farthest. The results showed that Heze population and Tai＇an population were relative independent populations, but there were still some gene exchange between five populations structure, Tai＇ an population should be given a high priority consideration germplasm conservation strategies owing to its highest genetic diversity Based on the study of population genetic in Salvia miltiorrhiza populations in situ

关 键 词： 丹参 标记 遗传多样性

领　　域： [生物学]