作　　者： ; ; ; ;

机构地区： 深圳职业技术学院应用化学与生物技术学院

出　　处： 《西北植物学报》 2008年第10期2055-2061,共7页

摘　　要： 为探讨鸡冠花热胁迫耐性与其SOD之间的关联,选用耐热品种Variety-Centrury Green 10叶期幼苗为试材,用二乙基二硫代氨基甲酸钠(DDTC)进行48h预处理,之后在45℃人工培养箱中进行热胁迫处理,观察其外观形态。结果表明,20mmol/LDDTC预处理显著抑制叶片SOD活性,明显减弱鸡冠花的热胁迫耐性,表现为幼苗热胁迫耐受时间显著缩短,弯颈、死亡率明显提高。在自然状态下,叶片中有1种MnSOD、1种Cu/ZnSOD和3种FeSOD;迁移率大小依次为Cu/ZnSOD>FeSOD>MnSOD;谱带强弱依次为FeSOD>Cu/ZnSOD>MnSOD。经热胁迫处理后,各种SOD同工酶条带亮度均呈现不同程度的增强-减弱的变化趋势,并诱导产生了1条新的Cu/Zn-SOD条带,与此同时MnSOD条带最先消失。由此推测,鸡冠花品种间耐热性差异与其SOD活性相关,与胁迫强度相对应,同时也与Cu/ZnSOD2的诱导产生相关联。 To investigate the relationship between the tolerance to high temperature stress and SOD in Celosia cristata, a cuhivars of Variety-Centrury Green which is strong tolerance to high temperature stress（in seeding with ten leaves）, were pulsed with 48 h DDTC pretreatment and subjected to high temperature stress under controlled conditions 45 ~C, then observed their external morphology. The results showed that SOD activity in leaves was remarkably inhibited when pulsed with 20 mmol/L DDTC pretreatment. The seedling of Variety-Centrury Green tolerance to high temperature stress was obviously decreased, which was evidenced by the obvious increase of bentneck rate,death rate and the shortness of the seedling tolerance time to high temperature stress. Three types of SOD were observed in nature state of the seeding. In these types,MnSOD and Cu/ZnSOD had one isozyme each and FeSOD had three. The turn of mobilities of them were Cu/ZnSOD〉FeSOD〉MnSOD;and bands activities were FeSOD）Cu/ZnSOD〉MnSOD. After the treatment of high temperature stress, all bands took on increase-decrease trend in different degree. Meantime, one new band named as Cu/ZnSOD was induced and MnSOD firstly disappeared. However, the newly induced band by high temperature stress mentioned above was inhibited by DDTC pretreatment. It is presumed that the tolerance to high temperature is highly related to SOD activity and is relative to stress strengthen,and the newly induced Cu/ZnSOD2 in Variety-Centrury Green.

关 键 词： 鸡冠花 热胁迫 同工酶 二乙基二硫代氨基甲酸钠

领　　域： [生物学]