作　　者： ; ; ;

机构地区： 中国农业科学院北京畜牧兽医研究所

出　　处： 《中国畜牧兽医》 2008年第10期82-85,共4页

摘　　要： 试验以猪为试验对象建立油酸-急性肺损伤模型。对油酸的注射剂量、宰杀方式进行比较优选;然后选取12头50日龄的猪,随机分为对照组和油酸组;处理后观察猪的呼吸状况,并在处理后不同时相点(1、3和6 h)宰杀猪,对肺组织的结构变化和肺含水量进行观察分析。结果表明,与对照组相比,注射适量油酸后,猪呼吸方式由胸腹式呼吸变为急促的腹式呼吸,呼吸频率明显升高;在注射后的3个时相点剖检发现,油酸组猪的肺组织出现典型急性肺损伤的病理形态。因此,在本试验条件下,以0.05 ml/kg体重,少量分次,耳静脉注射油酸成功建立了油酸-急性肺损伤模型,为进一步开展有关猪肺损伤及肺免疫防御的研究奠定了基础。 Experimental objective was to establish a.model of oleic acid （OA）- acute lung injury （ALI） in pigs. The injection dosage and slaying methods were compared and the excellent one was selected. Then twelve and fifty-day pigs were randomly divided into two groups - control group and OA group. The breathing status of pigs were observed. The pigs were slaughtered in 1, 3 and 6 h, and the changes of structure and the quantity of containing water of lung were observed and compared. After injecting OA, the breathing manner of pigs changed from thorax- abdominal to pressing abdominal breath, and the frequency of breath became higher. And there were typical ALI pathological changes in the OA group when the pigs were examined in 1, 3 and 6 h after injection. It is successful to establish the model of acute lung injure in pigs via intravenous injection of 0. 05 ml/kg avoirdupois OA by a few each time and more times. It contributes to the further study on porcine ALI and innate immune of the lung.

关 键 词： 猪 油酸 肺损伤 动物模型

领　　域： [农业科学] [农业科学] [农业科学]