作　　者： ; ; ; ;

机构地区： 肇庆学院化学化工学院

出　　处： 《化学研究与应用》 2008年第10期1335-1337,共3页

摘　　要： To establish an HPLC method for separation and determination of stigmasterol,β-sitosterol and lupeol in phyllanthus urinaria L by HPLC.A Eclipse Zorbax XDB-C18 column(4.6 mm×150 mm i.d.,5 μm) was used for chromatographic separation under the conditions with methanol as the mobile phase at flow rate of 0.7 mL/min.The detection wavelength was 210 nm.The results indicated that stigmasterol,β-sitosterol and lupeol could be baseline separated.The linear range of stigmasterol,β-sitosterol and lupeol were 0.4mg/mL～8.8 mg/mL(R2 were 0.9975),0.5～10.0 mg/mL(R2 = 0.9978) and 0.5mg/mL～10.0 mg/mL(R2 = 0.9981),respectively.The mean recoveries of stigmasterol,β-sitosterol and lupeol were 94.4%～95.1%,91.2%～97.9% and 92.4%～104.6 %,respectively.RSD was less than 5%.The method has been applied to monitor the extraction of stigmasterol,β-sitosterol and lupeol in phyllanthus urinaria L by super-critical fluid extraction with satisfactory. To establish an HPLC method for separation and determination of stigmasterol, β-sitosterol and lupeol in phyllanthus urinaria L by HPLC. A Eclipse Zorbax XDB-C18 column （4.6 mm × 150 mm i. d. , 5 μm） was used for chromatographic separation under the conditions with methanol as the mobile phase at flow rate of 0.7 mL/min. The detection wavelength was 210 nm. The results indicated that stigmasterol, β-sitosterol and lupeol could be baseline separated. The linear range of stigmasterol, β-sitosterol and lupeol were 0.4mg/mL ～ 8.8 mg/mL （R^2 were 0.9975 ）, 0.5 ～ 10.0 mg/mL （R^2 = 0.9978） and 0.5mg/mL - 10.0 mg/mL （R^2 = 0. 9981 ）, respectively. The mean recoveries of stigmasterol, β-sitosterol and lupeol were 94.4% -95.1%, 91.2% - 97.9% and 92.4% - 104.6 %, respectively. RSD was less than 5%. The method has been applied to monitor the extraction of stigmasterol, β-sitosterol and lupeol in phyllanthus urinaria L by super-critical fluid extraction with satisfactory.

关 键 词： 叶下珠 豆甾醇 谷甾醇 羽扇豆醇

领　　域： [理学] [理学]