作　　者： ; ; ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院

出　　处： 《动物学杂志》 2008年第5期25-30,共6页

摘　　要： 采用离体细胞培养诱导方法,研究微囊藻毒素-LR(microcystin-LR,MC-LR)对尼罗罗非鱼(Oreochromis niloticus)原代肝细胞的毒性效应。尼罗罗非鱼原代肝细胞经10、50、150、500μg/L MC-LR体外诱导24h后,单细胞微量凝胶电泳(SCGE)检测显示,与对照组相比处理组出现明显的彗星拖尾现象,说明MC-LR可引起尼罗罗非鱼肝细胞DNA的损伤,并随着剂量的增加,DNA的损伤程度增大。PI/Annexin V双染色流式细胞仪(FCM)检测表明MC-LR能明显引起肝细胞凋亡,与SCGE结果一致,且DNA损伤程度越大,细胞早期凋亡率越高,呈现明显的时间、剂量依赖性。本研究为进一步从分子、细胞水平阐明MC-LR的毒性以及致毒机理提供重要的理论依据。 The isolated hepatocytes of Nile Tilapia （ Oreochromis niloticus ） were exposed to MC-LR to study the mechanism of microcystin-LR （MC-LR） toxicity. After hepatocytes were exposed to 10,50,150,500 μg/L MC-LR for 24 h respectively, the single cell gel electrophoresis assay （SCGE） was used to investigate the effects on DNA. Induction of DNA strand break in all MC-LR exposed groups was significantly higher than that of the control group. Treatment time- and dose-dependent DNA migration caused by MC-LR and was observed. Flow cytometry （FCM） analysis indicated that the percentage of hepatocyte apoptosis increased in a treatment time- and dose-dependent manner. These results provide first experimental evidence showing the mechanism of MC-LR toxicity at cellular and molecular levels.

关 键 词： 微囊藻毒素 原代肝细胞 致毒机理 尼罗罗非鱼

领　　域： [生物学]