作　　者： ; ; ; ;

机构地区： 广西师范大学化学化工学院

出　　处： 《食品研究与开发》 2008年第10期84-87,共4页

摘　　要： 建立一个快速、简单、准确的固相萃取预处理高效液相色谱法测定罗汉果甜甙V含量的方法。样品经C18固相萃取管(200mg/3mL)纯化后以高效液相色谱检测。色谱柱为NH2P-50,流动相为V(乙腈)∶V(水)=85∶15,流速为1mL/min,检测波长为210nm。标准曲线自0.025g/L～1.000g/L呈线形关系(r2=0.9993)。研究表明:固相萃取法能很好的纯化罗汉果甜甙Ⅴ,回收率高达98.80%,RSD为0.52%。此法样品处理简单,溶剂消耗少,费用减少,分析结果重现性好,适于推广应用。 A high performance liquid chromatographic method was used for the determination of grosvenorii infusion which was treated by solid phase extraction.The sample was purified by C18 solid phase extraction tube and then detected by high performance hquid chromatography. The chromatographic column is NH2P-50, liquid phase is V：V=85：15, velocity of flow is 1 mL/min, wavelength of detection is 210 nm. The standard curve has linear relation during 0.025 g/L-1.000 g/L （r^2= 0. 999 3）. The results showed that： the method of solid phase extraction can well purify mogroside V, the average recovery rate of mogroside V was up to 98.80 %（RSD=0.52 %）.Compared to purification by macroporous resin,this method was simple, cheap, reliable. It can be applied broadly.

关 键 词： 罗汉果甜甙 固相萃取 高效液相色谱法

领　　域： [轻工技术与工程] [轻工技术与工程] [轻工技术与工程] [轻工技术与工程]