作　　者： ; ; ; ;

机构地区： 华南农业大学园艺学院

出　　处： 《氨基酸和生物资源》 2008年第3期11-15,共5页

摘　　要： 本文对GABA茶中有效成分γ-氨基丁酸的薄层扫描(TLC)检测以及提取、分离、纯化进行了研究,结果表明:正丁醇?醋酸?水体积比为4?1?1展开剂分离效果较好,R f值为0.46。采用双波长扫描法检测,扫描波长分别为λS=515nm,λR=680 nm,检测线性范围:0.5μL^15μL,样品平均回收率为98.75%。采用水提取法比乙醇提取法γ-氨基丁酸含量提高20%左右,732阳离子树脂对γ-氨基丁酸的静态最大吸附量为32.9 mg.g-1,1 h内其吸附速度较快,达吸附量的70%。样液pH值、流速等因子对树脂的吸附效率有影响,当pH值为3.0,流速3 m l.m in-1时,树脂的吸附量达到最大值。采用柠檬酸缓冲液和NH3.H2O进行洗脱,当pH为8.0~9.0时,γ-氨基丁酸洗脱率达94.68%。 The thin-layer chromatography （TLC） and extraction and purification of γ-aminobutyric acid of GABA tea were studied. The results showed that：l-butanol：acetic acid：water （4：1：1 ） was suited for the separation of γ-aminobutyric acid, Rf was 0.46. The scanning wave lengths were λS= 515nm, λR = 680nm, the linearity range was 0.5μL-15μL,and the average recovery rate was 98.75%. Water extraction was proved to be better than alcohol extraction with 20% higher γ-aminobutyric acid content. The maximum static adsorption quantity of 732 cation-exchange resin to γ-aminobutyric acid was 32.9mg/g. The adsorptive rate achieved a maximum of 70% adsorption quantity within the first lh. The pH and flow rate of sample liquid could influence the adsorption quantity, the optimal conditions were 3.0 （pH） and 3mlL·min^-1（flow rate）. Citric acid buffer and NH3·H2Owere fit to eluteγ-aminobutyric acid with 94.68% elution rate when the buffer pH was 8.0 - 9.0.

关 键 词： 氨基丁酸 测定 提取 分离 纯化

领　　域： [生物学]