作　　者： ; ; ; ; ; ; ; ;

机构地区： 中山大学中山医学院

出　　处： 《中山大学学报（医学科学版）》 2008年第5期566-569,574,共5页

摘　　要： 【目的】通过体内实验探讨人纤溶酶原Kringle 5(K5)对小鼠Lewis肺癌移植瘤生长与转移的抑制作用。【方法】采用腋下接种肿瘤细胞的方法建立Lewis肺癌细胞皮下种植瘤小鼠模型,分为PBS对照组和K5治疗组,观察各组肿瘤生长情况,测量肿瘤大小,制备小鼠体质量-时间曲线和肿瘤体积-时间曲线;采用皮下种植瘤剔除术建立Lewis肺癌自发性肺转移模型,术后分为PBS对照组和K5治疗组,观察各组小鼠肺组织转移瘤数量,取肺组织进行病理学分析。【结果】在皮下种植瘤模型中,与对照组相比,K5治疗组小鼠肿瘤体积-时间曲线变化平缓,肿块增长缓慢,肿瘤质量明显降低[分别为(4.57±0.79)g和(1.15±0.31)g,P=0.006];在Lewis肺癌转移瘤模型中,K5治疗组小鼠肺表面转移瘤结节数[分别为(15.75±9.79)个和(6.60±3.39)个,P=0.029]以及肺湿质量明显少于对照组,高倍镜下治疗组肺微转移灶数目亦较少。【结论】人纤溶酶原K5能显著抑制小鼠Lewis肺癌移植瘤的生长,并能明显抑制Lewis肺癌细胞转移。 [ Objective ] To investigate the inhibitory effects of human plasminogen kringle 5 （KS） on the growth and metastasis of mouse lewis lung carcinoma （LLC） in vivo. [Methods] To evaluate K5 activity in vivo, the mouse lung cancer graft model was established by injection of Lewis lung carcinoma cells subcutaneously into the oxter of C57BL/6J mice. On the 7th days after inoculation, the mice then received an intraperitoneal injection of K5 or phosphate buffered solution （PBS） as control. Tumor volume and mouse body weight were measured and plotted in tumor-volume curve and body-weight curve to estimate the tumor growth rate and general constitution of the animals during the course of treatment. A spontaneous metastasis mouse model of Lewis lung carcinoma was established by removing the primary tumors in the 7th days after inoculation. Right after the tumor remove, mice received K5 or PBS by intraperitoneal injection. The body weight and survival rate of both groups were recorded. After the mice were executed, the lungs were dissected, weighted, and undertaken a pathological analysis. [Results] The KS-treated group had a tumor growth rate which was significantly lower than that of the control group in grafted LLC mouse model, and distinctly depression of tumor weight [（4.57 ± 0.79） g, and （1.15 ± 0.31） g, respectively, P=0.006] and body weight. Compared to PBS-treated group, the incidence of metastasis to lung and the mean number of metastatic lung nodules （ 15.75 ± 9.79 and 6.60 ± 3.39 respectively, P=0.29） and the wet lung weight of each animal were reduced by K5 treatment in metastasis model of mouse Lewis lung carcinoma.Similarly, the pulmonary micrometastasis of K5-treated group decreased obviously with microscopic observation at high magnification. [Conclusion] K5 can inhibit the growth of Lewis lung carcinoma, and suppress the pulmonary metastasis of LLC cells in vivo.

关 键 词： 纤溶酶原 肺癌 肿瘤转移

领　　域： [生物学]