作　　者： ; ; ; ; ; ;

机构地区： 西北农林科技大学农学院

出　　处： 《中国农业科学》 2008年第9期2873-2880,共8页

摘　　要： 【目的】研究不同基因型绿豆主茎节位各功能叶片衰老与活性氧代谢特性,探讨绿豆叶片衰老机理,揭示绿豆产量形成的内部生理机制,为绿豆高产育种与栽培提供理论依据。【方法】以夏播区高产绿豆品种(系)冀绿2号、安9910和低产品种(系)泰来绿豆、赤峰绿豆为试验材料,测定开花—成熟期各功能叶片叶绿素、可溶性蛋白质含量、酶促防御系统的保护酶SOD、CAT活性和脂质过氧化产物MDA积累量等生理指标。【结果】绿豆开花后,随着衰老进程的推移,各功能叶片自下向上依次衰老,其叶绿素、可溶性蛋白质含量、SOD和CAT活性均呈下降趋势,MDA积累量持续上升。不同基因型间表现出明显差异,与低产品种(系)相比,高产品种(系)的叶片衰老缓慢,生育后期仍能维持相对较高的SOD、CAT活性。【结论】绿豆叶片衰老和活性氧代谢存在着基因型差异。SOD、CAT可能参与了绿豆叶片衰老进程的调控,并通过协同作用来保护叶片减轻活性氧伤害,使其功能期延长,产量提高。 [Objective] The study was tended to examine the aging and reactive oxygen metabolisms in the leaves of different mung bean genotypes at different nodes, explore the aging mechanisms of mung bean leaves, find out the internal yield-formation mechanisms of mung bean in order to provide a theoretical basis for high-yield breeding and production of mung bean. [Method] In the study, Jilu 2 and An9910, high-yielding varieties adopted in the summer planting region of mung bean, and Tailai mung bean and Chifeng mung bean, low-yielding varieties, were planted and the physiological indexes of their leaves, such as chlorophyll and soluble protein contents, SOD and CAT activities and MDA accumulation, were determined from flowering to ripening. [Result] After mung bean flowered, its functional leaves aged gradually from bottom to top, and the leaves showed decreased chlorophyll and soluble protein contents, decreased SOD and CAT activities and increased MDA accumulations as they grew old. There were significant differences in different genotypes of mung beans. Compared with the leaves of the low-yielding varieties, the leaves of the high-yielding varieties aged slower and SOD at CAT maintained higher activities at the late growth stage. [Conclusion] In mung bean, leaf aging and reactive oxygen metabolism vary depending on the genotypes. SOD and CAT probably involve in leaf-aging regulation and synergistically protect the leaves by abating the damage of reactive oxygen, as a result prolong the functional period of the leaves and raise the yield.

关 键 词： 绿豆 叶片衰老 活性氧代谢

领　　域： [农业科学] [农业科学]