作　　者： ; ; ; ; ;

机构地区： 西北农林科技大学园艺学院农业部西北园艺植物种质资源与遗传改良重点开放实验室

出　　处： 《西北植物学报》 2008年第7期1296-1302,共7页

摘　　要： 为了创制新的大白菜雄性不育材料,双酶切重组质粒pMD18-T-CMS7311-orf224和表达载体pWR306后,将CMS7311-orf224基因与线性表达载体pWR306进行定向连接,构建了细胞质雄性不育线粒体基因CMS7311-orf224的植物表达载体pWR-CMS7311-orf224,通过酶切和PCR验证pWR-CMS7311-orf224载体构建正确.采用快速冻融法,将表达载体导入农杆菌EHA105,转化大白菜材料06J28,对转化植株的GUS、PCR和RT-PCR检测表明,获得了2个大白菜转基因植株. In order to create a new cytoplasmic male sterile line of Chinese cabbage, the recombinant plasmid and expression vector pWR306 from double-enzyme digestion were linked directionally with the segments of CMS7311-orf224 gene to construct the plant expression vector pWR-CMS7311-orf224,which was intro duced into Agrobacterium strain EHA105 by means of rapid frozen thaw method. The results of PCR amplification and restriction digestion showed that the fragment of CMS7311-orf224 gene was introduced into pWR306 plasmid and the expression vector pWR-CMS7311-orf224 was transferred into Agrobacterium successfully. By GUS,PCR and RT-PCR preliminary testing,we obtained two 06J28 transgenic lines.

关 键 词： 大白菜 植物表达载体 遗传转化

领　　域： [生物学]