作　　者： ; ; ; (林晓坤）;

机构地区： 广东海洋大学水产学院

出　　处： 《广东海洋大学学报》 2008年第4期6-9,共4页

摘　　要： 克隆并测定了星洲银罗非鱼（Oreochromis sp．）细胞色素b基因（cytb）的全序列，得到全长1141bp的序列用于分析，用MEGA4．0分析软件与GenBank中的线粒体DNA序列进行序列比较，显示星洲银罗非鱼与其他鱼类的cytb基因的同源性较高，同源度介于83％～93％间；根据星洲银罗非鱼与莫桑比克罗非鱼、尼罗罗非鱼等12种鱼类的cytb基因序列构建的系统进化树，初步确定尼罗罗非鱼、莫桑比克罗非鱼为星洲银罗非鱼的父母本。 The complete sequence ofcyt b gene ofOreochromis sp. （ O. niloticus ×O. mossambicus ） was studied. 1 141bp sequence was obtained through PCR amplification, and was compared by MEGA 4.0 program with the mitochondrial DNA sequences downloaded from GenBank for comparison. The results showed that there was a close homology （83%--93%） for the cyt b gene sequence between Oreochromis sp. and other fishes. The phylogenetic tree was constructed based on cyt b gene of twelve fishes, such as Oreochromis sp., O. niloticus, 0. mossambicus etc. The parents （O. niloticus 9 , ♀. mossambicus ♂） and taxonomic position of Oreochromis sp. were initially affirmed through molecula data.

关 键 词： 星洲银罗非鱼 线粒体 细胞色素 基因 基因序列 分子系统学

领　　域： [生物学] [生物学]