作　　者： ; ; ; ; ; ; ;

机构地区： 中国水产科学研究院南海水产研究所

出　　处： 《海洋水产研究》 2008年第4期7-14,共8页

摘　　要： 运用组织学及组织化学的染色方法,对出膜后第1～44天(Days post-hatch,dph)的军曹鱼Rachycentron canadum头肾、脾脏和胸腺发育过程进行研究。首先观察到前肾管,3 d后,在肾管附近发现原始的造血干细胞,逐渐有红细胞和大淋巴细胞产生。之后,脾脏出现,其内毛细血管和红细胞丰富,还分布少量巨噬细胞。胸腺虽然是最后出现的淋巴器官,但是发育的非常快,内外分区明显。可以得出其淋巴器官出现顺序是头肾、脾脏和胸腺。在小淋巴细胞出现前,发现各淋巴器官内存在一些与非特异免疫机制相关的细胞,如巨噬细胞和网状细胞等。淋巴器官原基出现后开始淋巴化,其顺序是胸腺、头肾和脾脏。在采样的时间段里未观察到浆细胞,可能军曹鱼淋巴器官至此仍未发育成熟。 Histological and histochemical methods were applied to study the development of the head kidney, spleen and thymus in cobia Rachycentron canadum, during 1-44 day posthatch （dph）. The pronephric ducts were first observed, near which some primordial haemopoietic stem cells were observed 3 d later. The haemopoietic stem cells gradually differentiated into erythrocytes and large lymphocytes. The spleen developed later, and soon became rich in blood capillaries and erythrocytes, but had only a few macrophage. The thymus was the last lymphoid organ appeared but developed very quickly. An outer zone and inner zone in the thymus could be observed clearly. It was concluded that the sequence of appearance of lymphoid organs, from first to last, was head kidney, spleen and thymus. Before the small lymphocytes developed, cellular types involved in non-specific immunity mechanisms, such as macrophage and reticular cells, were observed in the lymphoid organs. The respective lymphoid organ anlage became thymus, head kidney and spleen successively. No plasma cells were observed in the samples, indicating that the lymphoid organs were not fully grown during this period.

关 键 词： 军曹鱼 淋巴器官 发育

领　　域： [生物学]