机构地区: 广东省农业科学院
出 处: 《动物营养学报》 2008年第4期435-441,共7页
摘 要: 选用初体均重为25.85g的军曹鱼幼鱼225尾随机分为5组,每组设3个重复,每个重复15尾。分别投喂添加5种不同剂量维生素C(添加量分别为0、37.5、75、150和300mg/kg)的试验饲料,试验期为56d,观察维生素C对军曹鱼幼鱼生长、组织维生素C积累量及非特异性免疫的影响。结果表明,随着饲料中维生素C添加量增加,军曹鱼幼鱼增重率和特定生长率呈现出上升后下降趋势,在75mg/kg时达到最高值,显著高于0和300mg/kg组(P<0.05)。全鱼粗蛋白质含量随着饲料中维生素C添加量的升高亦呈现先上升后下降趋势,在75mg/kg时达到最高值,全鱼干物质、粗蛋白质、粗脂肪、灰分含量在各组之间均无显著差异(P>0.05)。血清维生素C积累量随着饲料中维生素C添加量的增加呈现先上升后下降趋势,在150mg/kg时达到最高值且显著高于0mg/kg组(P<0.05);脑和肝脏中维生素C含量在300mg/kg时达到最高值。300mg/kg组脑中维生素C含量显著高于0,37.5和75mg/kg组(P<0.05),肝脏中维生素C含量显著高于0,37.5和150mg/kg组(P<0.05)。随着饲料中维生素C添加量的增加,溶菌酶活性呈现先上升后下降趋势,在75mg/kg达到最高,显著高于0,37.5和300mg/kg组(P<0.05);血清总抗氧化能力呈现上升趋势,75、150和300mg/kg组显著高于0mg/kg组(P<0.05);肾抗氧离子自由基在150mg/kg组达到最高,但各组之间无显著差异(P>0.05)。以鱼体增重率和血清溶菌酶含量为指标,经折线模型回归分析求得军曹鱼幼鱼饲料中维生素C的适宜添加量分别为70和80.72mg/kg。 The experiment was carried out to determine the canadum L. ). Two hundred and twenty five fish with an dietary vitamin C requirement of juvenile Cobia (Rachycentron average body weight of 25.85 g were randomly divided into 5 groups with 3 replicates and each replicate contained 15 fish; Five test forages containing different doses of vitamin C (0, 37.5, 75, 150, 300 mg/kg) were used to feed the fish for 56 days. The optimal dietary vitamin C requirement was evaluated based on growth performance, vitamin C accumulation contents in tissues and non-specific immunity in cobia juveniles. The results indicated that fish fed with 75 mg/kg of vitamin C had highest weight gain rate (WGR) and special gain rate (SGR), which were significantly higher than that of 0 and 300 mg/kg group(P(0.05). With the increase of dietary vitamin C, the crude protein content of whole fish increased at first and then decreased, which reached a peak at 75 mg/kg. But in each group, no significant difference was obtained in the content of dry matter, crude protein, crude fat and crude ash of whole body (P〉0.05). With the increase of the level of dietary vitamin C, vitamin C accumulations of serum in fish increased and reached a peak, and then decreased, and the highest vitamin C accumulations of serum were found in the fish fed with 150 mg/kg of vitamin C, which was significantly higher that of 0 mg/kg group(P〈0.05). The vitamin C accumulations in liver and brain of fish fed with 300 mg/kg of vitamin C were the highest. The vitamin C accumulations in the brain of 300 mg/kg group were significantly higher than that of 0, 37.5 and 75 mg/kg groups(P〈0.05), and the vitamin C accumulations in the liver were significantly higher than that of 0, 37.5 and 150 mg/kg groups(P〈0.05). Lysozyme increased at first and then decreased with the increase of the level of dietary vitamin C, and the lysozyme in 75 mg/kg group was the highest, which was significantly higher than that of 0, 37. 5 and 300 mg/kg groups. T