机构地区: 华南理工大学
出 处: 《中国动脉硬化杂志》 2008年第7期519-522,共4页
摘 要: 目的探讨替米沙坦和血管紧张素Ⅱ对乳鼠胸主动脉平滑肌血管紧张素转化酶2表达的影响。方法应用蛋白免疫印迹法检测血管紧张素转化酶2蛋白的表达,以逆转录聚合酶链反应法检测血管紧张素转化酶2mRNA表达。结果在10-8~10-5mol/L浓度范围内,替米沙坦呈浓度依赖性上调血管紧张素转化酶2蛋白的表达,在0~24h时间内,10-6mol/L替米沙坦呈时间依赖性促进血管紧张素转化酶2的蛋白表达,并促进血管紧张素转化酶2mRNA的表达;在10-8~10-5mol/L浓度范围内,血管紧张素Ⅱ呈浓度依赖性下调血管紧张素转化酶2蛋白的表达;10-6mol/L替米沙坦能明显地拮抗10-6mol/L血管紧张素Ⅱ对血管紧张素转化酶2蛋白和mRNA表达的抑制作用。结论替米沙坦能促进胸主动脉平滑肌血管紧张素转化酶2蛋白和mRNA的表达,并能明显拮抗血管紧张素Ⅱ对血管紧张素转化酶2蛋白和mRNA表达的抑制作用。 Aim To explore effects of telmisartan and angiotensin Ⅱ )Ang Ⅱ ) on expression of angiotensin converting enzyme-2 ) ACE-2) in thoracic aortic smooth muscle cells ) A10 cells), Methods The expression of ACE-2 protein was tested by Western blotting analysis and the expression of ACE-2 mRNA was determined with reverse transcription-polymerase chain reaction(RT-PCR) technique. Results Telmisartan at the concentration from 10^-8 to 10^-5 mol/L dose-dependently enhanced the expression of ACE-2 protein; within 0 to 24 h, 10^-6 mol/L telmisartan increased ACE-2 protein expression in a time-dependent manner. Moreover, telmisartan at 10^-6 mol/L upregulated the expression of ACE-2 mRNA. AngⅡ at 10^-8 to 10^-5 mol/ L downregulated the expression of ACE-2 protein in a dose-dependent manner. Telmisartan at 10^-6 mol/L obviously blocked the inhibition of 10^-6 mol/L Ang Ⅱ on the expression of ACE-2 protein and ACE-2 mRNA respectively. Conclusion Telmisartan not only up-regulates the expression of both ACE-2 protein and ACE-2 mRNA in A10 cells, but also antagonizes the inhibition of Ang Ⅱ on the expression of both ACE-2 protein and ACE-2 mRNA.
关 键 词: 生理学 血管紧张素转化酶 替米沙坦 血管肾张素 胸主动脉平滑肌细胞
领 域: [生物学]