机构地区: 广州大学化学化工学院食品系
出 处: 《食品科技》 2008年第8期31-34,共4页
摘 要: 以脱脂豆粕为原料,确定用木瓜蛋白酶制备大豆多肽的酶解工艺条件,并初步明确大豆蛋白酶解产物与锌(Zn)发生配合作用生成锌-多肽配合物的技术条件。结果表明:大豆蛋白达到最大水解度的工艺条件是pH为8.0,水解温度为55℃,水解时间为120min,E/S为300U/g;影响配合反应及配合物得率、锌配合率的技术条件是多肽与锌的质量比为3∶1,pH6.0,温度为70℃,反应时间为40min。 Enzymatic hydrolysis condition of bean cake protein was investigated. Preparation technology of Zn ( Ⅱ )-peptides complexes from zinc chloride and bean cake hydrolysates by papain was studied. The results showed that the best hydrolysis condition was pH 8.0, 55 ℃, 20 min and enzyme/substrate 300 U/g. The best preparation technology condition of Zn-peptides complex was that the mass ratio of soybean peptide to zinc was 3:1, pH6.0, 70 ℃, and the complexes reaction time was 40 min.