作　　者： ; ; ; ; ; ;

机构地区： 华南农业大学生命科学学院

出　　处： 《热带亚热带植物学报》 2008年第4期334-338,共5页

摘　　要： 在海藻酸钠-壳聚糖固定化木瓜蛋白酶(immobilized papain on sodium alginate-chitosan,IPSAC)的实验中,当给酶量为1mgg-1载体时,酶活性为39.2U,酶活力回收为21.1%。在尼龙布固定化木瓜蛋白酶(immobilized papain on nylon,IPN)的实验中,当每块尼龙布(3cm×3cm)给酶量为1mg时,酶活性为35.6U,酶活力回收为19.2%。木瓜蛋白酶(papain,PA)、IPSAC、IPN的最适pH分别为7.2、7.2和6.8。PA及IPSAC在70℃以下活性稳定;IPN在50℃以下活性稳定。IPSAC与IPN半衰期分别为59d和66d。 Papain was immobilized on sodium alginate-hitosan and nylon, respectively. The immobiliation conditions and characterization of the immobilizde enzyme were studied. When 1 mg g^-1 carrier papain （PA） was loaded on sodium alginate-chitosan （IPSAC）, the IPSAC activity was 39.2 U, and the recovery of activity was 21.1%. As PA was loaded 1 mg on one block nylon （3 cm × 3 cm）, the IPN activity was 35.6 U, and the recovery of activity was 19.2%. The optimum pH of PA, IPSAC and IPN were 7.2, 7.2 and 6.8, respectively. PA and IPSAC were stable when the temperature was lower than 70℃, while IPN was stable below 50℃. The half- life of IPSAC and IPN were 59 d and 66 d, respectively.

关 键 词： 木瓜蛋白酶 固定化酶 海藻酸钠 尼龙布

领　　域： [生物学]