作　　者： ; ; ; ; ; ; ;

机构地区： 中山大学附属第一医院

出　　处： 《心脏杂志》 2008年第4期420-423,共4页

摘　　要： 目的探讨血管紧张素(Ang)-(1-7)对急性心肌缺血/再灌损伤(ischemic-reperfusion injury,IRI)家兔内皮功能的影响。方法建立家兔急性心肌IRI模型,将45只新西兰大白兔随机分为假手术组、IRI模型对照组及Ang-(1-7)组。分别于缺血/再灌注前后检测各组静脉血中一氧化氮(NO)、内皮素-1(ET-1)的含量。超声测量各组家兔肱动脉内皮依赖性舒张功能EDD(%)的变化。结果模型对照组NO的含量及EDD(%)值较假手术组明显降低(P<0.05),ET-1的含量明显增高;Ang-(1-7)组NO含量及EDD(%)值较模型对照组明显升高(P<0.05),ET-1含量较对照组明显下降(P<0.05)。NO的含量与EDD(%)值有良好的相关性。结论Ang-(1-7)预处理可以改善急性IRI期家兔内皮功能,肱动脉EDD是一种评价IRI家兔内皮功能的良好指标。 AIM To explore the effects of Ang- （ 1 - 7） on endothelial dysfunction in rabbits with ischemic- reperfusion injury（IRI）. METHODS Myocardial ischemic reperfusion injury models were set up by ligating and loosening rabbits＇ coronary artery. Forty-five male New Zealand rabbits were randomly divided into sham-operation group, model control group and Ang-（ 1-7 ） group, which were treated respectively with intravenous infusion of Ang-（ 1- 7 ） （25 mg/kg per hour） or saline after operation. The levels of plasma NO and endothelin-1 （ET-1） were determined under quiescent condition before and after the reperfusion. The endothelium dependent dilation （EDD （%））, that would represent the endothelial function, was evaluated with the aid of uhrasonoscope. RESULTS The levels of plasma NO and EDD （%） in model control group were significantly lower than those in sham-operation group（ P 〈 0. 05 ）. The level of ET-1 was significantly higher than that in model control group（ P 〈0. 05 ）. Compared with those in model control group, the levels of NO and EDD（ % ） in Ang-（ 1- 7 ） group were significantly higher （P 〈 0.05 ） and ET-1 was lower （P 〈 0. 05 ）. A positive correlation was found between NO level and EDD（ % ） value and a negative correlation was found between ET-1 level and EDD （%） value. CONCLUSION Pretreatment with Ang- （ 1 - 7） improves vascular endothelial function at acute stage in rabbits suffering from IRI, which can be appropriately evaluated by brachial artery＇s EDD.

关 键 词： 血管紧张素 缺血 再灌注损伤 内皮功能 内皮依耐性舒张

领　　域： [生物学]