作　　者： ; ; ; ; ; (赖志勇）;

机构地区： 广东省农业科学院

出　　处： 《华中农业大学学报》 2008年第3期445-449,共5页

摘　　要： 应用高效液相色谱（HPLC）法测定了黄皮、杨梅果肉中的绿原酸、表儿茶素和芦丁的含量。色谱柱为Nucleosil C18反相柱（250 mm×4 mm）,流动相为20%的甲醇（A）,30%乙腈（B）,pH 3.2,梯度洗脱,流速0.8mL/min,检测波长280 nm,柱温35℃。结果表明：3种标准品的线性范围为0.390 6～50.000 0μg/mL,相关系数r=0.999 9。标准品在0～48 h内经多次测定,保留时间和峰面积的相对标准偏差分别为0.78%～0.91%和0.67%～0.95%;回收率和回收率相对标准偏差分别为93.21%～103.50%和2.77%～3.94%;仪器最低检出限分别为0.090 1、0.095 5、0.098 0μg/mL。测定结果还表明：黄皮和杨梅果肉中均含有一定量的绿原酸、表儿茶素和芦丁。黄皮的4个品种中,3种物质的含量（鲜重）分别为4.046 8～38.669 0、4.316 4～84.614 8、52.392 4～130.609 7μg/g;杨梅的4个品种含量（鲜重）分别为17.564 4～93.546 3、113.090 8～236.401 5、46.366 7～168.370 0μg/g。 A high-performance liquid chromatography（HPLC） method to analyze chrologenic acid, epicatechin and rutin in wampee and wax myrtle flesh has been developed. Samples were injected into reversed-phase Nucleosil C18 column（250 mm× 4 mm）. Mobile phase A was 20%0 methanol and mobile phase B was 30% acetonitrile, pH was adjusted to 3. 2. Gradient elution proceeded at the flow rate 0. 8 mL/min. Operation conditions were as follows, column temperature 35 ℃, detection at 280 nm. The results showed that： Linear calibration of three standard samples were within 0. 390 6-50. 000 0 μg/mL with correlation 0. 999 9. Relative standard deviation（RSD） of run time and peak area were respectively within 0. 78% -0. 91 % and 0. 67 -0. 95 % after standard samples were repeated analyzed in 48 h. Recovery was within 93.21%-103. 50% with RSD of 2. 77%-3. 94%. The limit of detection （LOD） were 0. 090 1, 0. 095 5, 0. 098 04μg/mL. The method was validated for chrologenic acid, epicatechin and rutin present in flesh of wampee and wax myrtle. The contents of those three substances were within 4. 046 8-38. 669 0, 4. 316 4-84. 614 8 , 52. 392 4-130. 609 7μg/g fresh weight in four wampee varieties, and within 17. 564 4-93. 546 3, 113. 090 8-236. 401 5, 46. 366 7-168. 370 0 μg/g fresh weight in four wax myrtle varieties.

关 键 词： 高效液相色谱 黄皮 杨梅 绿原酸 表儿茶素 芦丁

领　　域： [农业科学] [农业科学]