作　　者： ; ; ; ; ; ; ;

机构地区： 西北农林科技大学资源环境学院

出　　处： 《西北农林科技大学学报（自然科学版）》 2008年第7期179-184,共6页

摘　　要： 【目的】研究W/O十六烷基三甲基溴化铵(CTAB)微乳液体系和HAc-NaAc缓冲液体系中,m-6菌株固态发酵后产生的胞外木质素降解酶漆酶(Lac)、木质素过氧化物酶(Lip)、锰过氧化物酶(Mnp)酶促反应的最佳反应条件。【方法】通过分别对Lip(以2,2′-连氮-二(3-乙苯基并噻唑-磺酸)为底物)、Mnp(以MnSO4为底物)和Lac(以藜芦醇为底物)3种木质素降解酶在W/O型CTAB微乳液体系和HAc-NaAc缓冲液体系中的酶活测定,研究不同温度、pH和底物浓度对酶促反应的影响,探讨最佳反应条件,并比较两种反应体系下的酶活。【结果】在W/O型CTAB微乳液体系中,Lip、Mnp和Lac 3种酶酶促反应的最佳条件为:温度37℃、pH分别为4.5,4.5和3.5;在HAc-NaAc缓冲液体系中,3种酶酶促反应的最佳反应条件为:温度37℃,pH分别为5.0,5.0和4.0;在两种反应体系中的最佳反应底物浓度相同,即藜芦醇、MnSO4、2,2′-连氮-二(3-乙苯基并噻唑-磺酸)浓度分别为0.053,0.116,0.492 mmol/L。在W/O型CTAB微乳液中,Lip和Mnp酶活比其在HAc-NaAc缓冲液体系中分别提高了81.45%和36.75%,但Lac酶活却减少了2.914倍。【结论】得到了Lac、Lip和Mnp酶3种木质素降解酶在W/O型CTAB微乳液体系和HAc-NaAc缓冲液体系中的最佳反应条件,为木质纤维素的生物降解奠定了基础。 [Objective] The reaction conditions of oxidase （Lip）,Manganese （Mnp） and Laccase （Lac）, ligninolytic enzyme system, which contain lignin per were investigated in W/O microemulsion of centyltri methy ammonium bromide （CTAB） （W/O CTAB ME） and HAc-NaAc buffer solution （HAc-NaAc BS）. The enzymes were extracted from white-rot fungi m-6 which were cultured from solid fermentation. [Method] Through measuring the Lip （3,4-Dimethoxybenzyl alcohol as its substrate）,Mnp （MnSO4 as its substrate） and Lac （2,2＇-azino-bis （3-et hylbenzthiazoline-6-sulp-honic acid （ABTS） as its substrate） activities in W/O CTAB ME and HAc-NaAc BS, the factors which affect ligninolytic enzyme activity could be found to study the optimum reactive conditions of ligninolytic enzyme system,and the enzymes＇ activities in these,two different systems were compared. [Result] In W/O CTAB ME, the optimal temperature was 37℃ and the optimal pH was 4.5,4.5 and 3.5 for Lip,Mnp and Lac respectively. In HAc-NaAc BS,the optimal temperature was also 37℃ and the optimal pH was 5.0,5.0 and 4.0 for Lip,Mnp and Lac respectively;The optimal substrate concentration was 0. 053,0. 116 and 0. 492 mmol/L for Lip,Mnp and Lac respective ly. The reasults showed that the Lip and Mnp activitives were improved 81.45% and 36.75% ,while the Lac activity decreased 2. 914 times. [Conclusion] The optimal reaction conditions of Lac,Mnp and Lip in W/O CTAB ME and HAc-NaAc BS were found,and they will do some contribution to lignin biodegradation.

关 键 词： 木质素降解酶 菌株 型微乳液 酶活性 漆酶 木质素过氧化物酶 锰过氧化物酶

领　　域： [生物学]