作　　者： ; ; ; ; ; ;

机构地区： 暨南大学生命科学技术学院

出　　处： 《生态毒理学报》 2008年第3期250-255,共6页

摘　　要： 为从分子水平上研究II相去毒酶谷胱甘肽S-转移酶(Glutathione S-transferase,GST)在斜带石斑鱼(Epinephelus coioides)等海水鱼类机体内代谢去毒过程中的作用及机制,采用RT-PCR及RACE法,分离、克隆得到斜带石斑鱼肝脏alpha-GST(GSTA)、rho-GST(GSTR)基因cDNA全序列.序列分析结果表明,斜带石斑鱼肝脏GSTA基因cDNA全长1008bp,编码223个氨基酸;GSTR基因cDNA全长1002bp,编码225个氨基酸.构建系统进化树发现,斜带石斑鱼与鲤鱼、斑马鱼GSTA氨基酸同源性较高,而GSTR为鱼类所特有并共同占据进化树上独立的分枝,与大鼠、小鼠、人等哺乳动物GST现有所有类型同源性均较低. To research the molecular mechanism of phase II detoxifizyme （glutathione S-transferases, GST） in the metabolism of toxics in marine fish, we cloned the fall-length cDNA sequences of alpha-GST （GSTA） and rho-class GST （GSTR） genes from the liver of orange-spotted grouper （Epinephelus coioides） by RT-PCR and RACE methods. The sequence analysis showed that the complete GST cDNA sequence of GSTA of orange-spotted grouper was 1008bp in length, containing a coding region of 672bp （encoding 223 amino acids）. And the complete cDNA sequence of GSTR was 1002bp in length, containing a coding region of 678bp （encoding 225 amino acids）. Results of the phylogenetic analysis revealed that the deduced amino acid sequence of the orange-spotted grouper was very similar to the GSTA of other fishes, such as common carp and zebrafish. The GSTR existed only in fish, but not in any other aquatic animals or land mammals （e.g. rat, mouse and human）.

关 键 词： 斜带石斑鱼 谷胱甘肽 转移酶 基因克隆 序列分析

领　　域： [生物学]