作　　者： ; ; ;

机构地区： 中国农业科学院北京畜牧兽医研究所

出　　处： 《农业生物技术学报》 2008年第3期412-416,共5页

摘　　要： 骨桥蛋白(osteopontin,OPN)基因是近年来在猪繁殖性状上发现的一个候选基因。实验以长白猪,大白猪和北京黑猪为研究对象,研究OPN基因多态性与猪繁殖性状之间的关联。经特异性产物的扩增并结合测序发现,OPN基因的第6内含子存在305bp的缺失突变而产生多态。在长白猪和大白猪中发现了AA、AB和BB3种基因型,均处于中度多态,且已达到Hardy-Weinberg平衡状态;在北京黑猪群体中没有多态,只有BB基因型。利用SAS8.2软件采用最小二乘法拟合线性模型,将不同基因型与总产仔数(TNB)、产活仔数(NBA)和初生重(WB)进行了关联分析,结果表明,长白猪初产母猪AA型个体的TNB和NBA显著高于AB和BB型个体(P<0.05),经产母猪AB型个体的TNB和NBA显著高于AA型与BB型个体(P<0.05),A等位基因对TNB、NBA和WB均表现为正效应。在大白猪中,不论是初产母猪还是经产母猪,BB和AB型个体的TNB和NBA高于AA型个体,但差异不显著(P>0.05),B等位基因对TNB和NBA都表现为正效应。 Osteopontin （OPN） gene was regarded as a candidate gene for pig reproductive traits. In this experiment, the relationship between genetic polymorphism of OPN gene and reproductive traits was studied in Landrace pig, Large White pig and Beijing Black pig. The polymorphism was found by the PCR, which was induced by the deletion mutation of 305 bp in the sixth intron of OPN, gene after sequencing. AA, AB and BB genotypes were found in Landrace pig and Large White pig populations, which showed a moderate polymorphism, and statistical results of χ^2-test indicated that this locus fitted Hardy-Weinberg equilibrium;While this polymorphism was not found in Beijing Black pig, and only BB genotype present in it. Meanwhile, effect of polymorphism for OPN gene on total number born （TNB）, number born alive （NBA） and born weight （WB） were analyzed. Results indicated that the first parity Landrace pig with AA genotype showed more TNB and NBA than that with AB and BB genotype significanfly（P 〈 0.05）, the individual of multiparous sows with AB genotype showed more TNB and NBA than that with AA and BB genotype significantly （P 〈 0.05）, and A allele had the positive effective on TNB, NBA and WB. While in large White pig, the TNB and NBA of sow with BB and AB genotype were more than those with genotype AA, but the results showed no significant difference （P 〉 0.05）, and B allele had the positive effective on TNB and NBA.

关 键 词： 猪 基因 多态性 繁殖性状 关联分析

领　　域： [农业科学]