作　　者： ; ; ; ;

机构地区： 广东海洋大学水产学院广东省水产经济动物病原生物学及流行病学重点实验室

出　　处： 《广东海洋大学学报》 2008年第3期88-92,共5页

摘　　要： 应用PCR技术扩增16S rRNA基因和amoA(ammonia monooxygenase subunit A)的基因片段,并测定其序列,对一株源于海水养殖水体的高效氨氧化细菌(ammonia-oxidizing bacteria,AOB)进行了系统发育分析。结果表明,经PCR扩增得到了1 098 bp的16S rRNA基因片段和491 bp的amoA基因片段,将其序列用NCBI-Blast软件在GenBank数据库中进行同源性检索后发现,该菌株的16S rRNA基因序列和amoA基因序列分别与亚硝化单胞菌Nitrosomonas sp.NS20的相对应基因片段相似性分别为98.4%和96.7%。在此基础上构建了系统发育树,表明用该菌株的16S rRNA基因片段和amoA基因片段构建的系统发育树均与亚硝化单胞菌属类聚在一起,结合该菌株形态和生理生化特性,鉴定该株氨氧化细菌属亚硝化单胞菌。 A strain of marine ammonia-oxidizing bacterium （AOB）, N2005 isolated from marine aquaculture water was phylogenetic analyzed. Total DNA of the bacteria was extracted, and the fragments of the 16S rRNA gene and ammonia monooxygenase subunit A（amoA） gene were amplified by polymerase chain reaction （PCR）. The amplified fragments of 16S rRNA gene and amoA gene were 1098 bp and 491 bp, respectively. Homology was analyzed by searching in the GenBank with NCBI-Blast software. The results showed that the amplified fragments had high homology with the 16S rRNA gene and amoA gene of Nitrosomonas sp. NS20 （98% and 96%, respectively）. Based on the 16S rRNA and amoA sequence, phylogenetic tree of the system was constructed, showing that the ammonia-oxidizing bacterium in present study should belong to Nitrosomonas.

关 键 词： 氨氧化细菌 聚合酶链反应 同源性分析

领　　域： [环境科学与工程] [农业科学] [农业科学] [农业科学]