作　　者： ; ; ; ; ; (邓岳松）;

机构地区： 中国水产科学研究院珠江水产研究所

出　　处： 《渔业现代化》 2008年第3期27-30,46,共5页

摘　　要： 为了验证耐高温酶制剂的使用效果,试验在基础饲料中添加0.1%的耐高温饲用酶,饲养奥尼罗非鱼60d,测定罗非鱼血清中非特异免疫指标,并电镜观察小肠细胞微绒毛排列情况;同时测定饲料厂制粒前后蛋白酶活力的变化情况。结果表明,添加耐高温酶制剂组超氧化物歧化酶(SOD)和溶菌酶水平比对照组有显著提高(P<0.05);同时,试验组罗非鱼小肠细胞微绒毛排列整齐、细而长,吸收面积增大;对照组的小肠细胞的微绒毛稀疏、短,排列不均匀。结果证实,在罗非鱼饲料中添加耐高温酶可以显著增强其免疫力,增加其对饲料的吸收率。制粒冷却包装后酶活力仍可保持90%以上,而普通酶只有25%左右。 Enzymes are proteins, most of them can not tolerant high temperature above 70 ℃. A 60-day experiment was conducted to evaluate the use of the thermostability enzyme on immunity and microvilli lying the gut of tilapia. And the protease activity of the feed was determined before and after pelleting. The thermostability enzyme （JM301） was supplemented in the basic diet with the level of 0.1%. The results showed that the addition of the special enzyme had positive effects on the Superoxide dismutase and Lysozyme （P 〈 0.05）. Through the tele-microscope, the microvilli lying the gut of the thermostability enzyme group was tidier, thinner and longer than control group, which indicated that the absorb area became bigger than the control group. The protease activity could still maintain more than 90% after pelleting of the thermostability enzyme, while the common enzyme only could maintain about 25%. The results of the experiment indicated that supplementation of the thermostability enzyme in the feed could promote the non-immunology function and increase the absorption rate.

关 键 词： 罗非鱼 耐高温酶制剂 免疫 免疫血清 超氧化物歧化酶 溶菌酶

领　　域： [农业科学] [农业科学] [农业科学] [农业科学]