作　　者： ; ; ; ; ; ; ; ;

机构地区： 广东省农业科学院

出　　处： 《食品与生物技术学报》 2008年第3期88-93,共6页

摘　　要： 探索了不同的培养条件下毛云芝菌产漆酶的情况,发现该菌漆酶属于组成型漆酶,其酶活力高,产酶周期短,酶的合成与菌体生长没有明显相关性;以漆酶的酶活为指标,采用正交试验设计方法对毛云芝菌液体培养的摇瓶生长条件进行了优化研究。结果表明:优化培养基的组成(质量分数)为麸皮3%,蛋白胨0.2%,牛肉膏0.3%,MgSO4.7H2O 0.05%,KH2PO40.3%;TE6%(体积分数),VB120 mg/L;自来水,pH自然。该营养条件下的酶活为1 668 U/mL,约是优化前培养基酶活的16倍,为工业化生产提供了一定的理论依据。 In this manuscript, the liquid culture conditions for laccase production by Coriolus hirsutus were investigated. The fungi could constitutively produce laccase with high enzyme activity during short fermentation time. There was no correlation between the laccase production and mycelium growth. The submerged culture condition for laccase production by Coriolus hirsutus was optimized by orthogonal test. The result showed that the optimal medium composition was as follows： wheat bran 3%, peptone 0. 2%, beef extract 0.3%, MgSO4 · 7H2O 0.05%, KH2PO4 0.3%, TE 6%, VB1 20 mg/L, tap water, natural pH value. Enzyme activity under the optimal culture conditions was 1668 U/mL which was 16 fold of that of the original medium.

关 键 词： 毛云芝菌 液体培养 优化 酶活

领　　域： [生物学] [生物学]