作　　者： ; ; ; ;

机构地区： 南昌大学

出　　处： 《分析试验室》 2008年第6期47-51,共5页

摘　　要： 以ODS为参比,研究了5种水溶性维生素和5种核苷在姜黄素键合硅胶固定相(CCSP)上的色谱行为,考察了甲醇、流动相pH和离子强度对CCSP分离这类极性化合物的影响,探讨了该新固定相的色谱保留机理。结果表明,在V(甲醇)∶V(0.01 mol/L NaH2PO4)=40∶60(pH 3.5)流动相体系中,5种水溶性维生素在CCSP上实现较好分离;核苷的分离则是在以纯水为流动相的条件下实现的。与ODS柱相比,在相同条件下,CCSP对5种水溶性维生素具有较高的选择性,洗脱顺序与ODS具有显著差异,且保留明显比ODS强(B6除外);与此相反,相同色谱条件下,CCSP对核苷的保留比ODS弱,CCSP可实现核苷的简便有效分离,同时胸苷与黄苷的洗脱顺序也与ODS不同。以上说明不同保留机理的存在,CCSP具有典型的反相色谱特征,但疏水性比ODS弱;极性的配体使CCSP在对溶质的分离分析中除疏水作用外,还存在n-π和π-π作用、氢键作用、偶极-偶极等作用;协同作用的结果使CCSP在水溶性极性化合物的分离分析中显示出优势。 A new curcumin bonded silica gel stationary phase （CCSP） was used for vitamins and five nucleosides by HPLC. The high-performance liquid chromatograph the separation of five water-soluble ic behavior of the solutes was studied in comparison with conventional ODS. The influence of mobile phase variation such as methanol content, pH and ionic strength on the retention behavior was investigated. And the retention mechanism of these solutes on CCSP was also discussed. The results indicated that a better separation of five water-soluble vitamins was achieved on CCSP using methanol-0.01 mol/L NaH2 PO4 （40： 60, v/v, pH 3.5） as mobile phases, and the separation of nucleosides was carfled out just with water as mobile phase. Compared with ODS, on one hand, CCSP had much better separation selectivity for vitamins and the elution order of five vitamins was very different from that on ODS, additionally, four of them exhibited stronger affinities to CCSP. On the other hand, though the retention of five nucleosides on CCSP was not as long as that on ODS under the same chromatographic conditions CCSP could achieved effective separation of nucleosides with different selectivity for thymidine and xanthosine from ODS. According to the chromatographic data, we could conclude that different retention mechanisms existed in the chromatographic processes of the two stationary phases. CCSP behaved as a reversed-phase packing with weaker hydrophobicity, owing to its polar ligand, various chromatographic retention mechanisms were responsible for the separation of above compounds, such as hydrophobic interaction, n-π and π-π interaction, hydrogen bonding interaction, and dipole-dipole interaction. CCSP had advantages for the separation of water-soluble polar compounds over ODS because of synergistic mechanisms.

关 键 词： 反相高效液相色谱法 姜黄素键合硅胶固定相 水溶性维生素 核苷 色谱保留机理

领　　域： [理学] [理学]