作　　者： ; ; ; ; ;

机构地区： 中国水产科学研究院南海水产研究所

出　　处： 《南方水产》 2005年第6期1-7,共7页

摘　　要： 对RAPD标记在合浦珠母贝F1代全同胞家系共27个样品中的分离方式进行了研究。从21条RAPD随机引物中筛选了8条引物用于扩增分析,结果共扩增出49个位点,其中正常位点47个(95.9%),异常位点2个(4.1%),即子代有扩增带而双亲无。47个正常位点中3个位点在F1中不分离(6.4%),44个位点发生分离(93.6%,即多态位点)。卡方检验表明,44个分离位点中32个符合孟德尔分离规律(72.7%),12个位点发生偏分离(27.3%)。符合孟德尔分离位点中按11分离位点占43.8%,31分离位点占56.2%,其中,父本特有标记、母本特有标记和双亲共有标记分别占28.2%,15.6%和56.2%,父本特有标记略多于母本特有标记。根据“双假测交”理论,11分离位点是构建F1代家系的作图位点。较高的11分离位点表明,利用RAPD标记构建合浦珠母贝遗传连锁图谱是可行的。 The mode of segregation of random amplified polymorphic DNA （RAPD） markers in 27 F1 full siblings of Pinctada fucata from a single pair of parents was studied using eight 10-mer primers screened from 21. Of the total 49 loci amplified, 47 loci are normal, accounting for 95.9%, and two loci are unexpectedly amplified in F1 but not in parents. There are 44 loci segregating in the F1 individuals, accounting for 93.6%. Chi square tests indicate that the segregation of 12 loci are deviated significantly from Mendelian fashion, while the segregation of 32 loci are in line with Mendelian fashion, among which 1 ： 1 segregating loci account for 43.8% and 3 ： 1 segregating loci 56. 2%. Among the 32 Mendelian segregation loci, paternal specific, maternal specific and biparental loci account for 28.2%, 15.6% 和 56. 2%, respectively, indicating that the number of male specific markers is slightly larger than that of female specific markers. The high percentage of 1 ：1 segregating loci revealed in this study indicates that RAPD markers are useful in construction of linkage map of P.fucata using F1 family materials based on double pseudo-testcross configuration.

关 键 词： 全同胞家系 代 孟德尔遗传 基因型 多态性

领　　域： [生物学]