作　　者： ; ; ; ;

机构地区： 中国水产科学研究院南海水产研究所

出　　处： 《南方水产》 2005年第2期35-40,共6页

摘　　要： 研究副溶血弧菌对斑马鱼的感染模式。试验以注射、创伤、浸泡等3种不同的感染方式分2次进行,每组10尾斑马鱼,结果显示注射感染只在高浓度菌液时有死亡,48h的LC50是5.63×107CFU·mL-1,而创伤感染偶有死亡,浸泡感染则未见死亡,表明注射感染是斑马鱼副溶血弧菌的重要感染模式。同时注射感染结果也显示低水温的死亡率远远低于高水温的死亡率,表明适宜的温度和弧菌的浓度是其致病的关键。并初步探讨了大豆异黄酮作为饲料添加剂的免疫增强作用,各组试验鱼分别投喂黄酮含量为0.5%、1%、2%的饲料,喂养两周后开始注射3.6×109CFU·mL-1的副溶血弧菌悬液,以投喂1%的试验组的抗感染效果较好,表明其对减少养殖病害的发生有一定的好处,为养殖生产提供一种新的免疫增强剂。 To study the infection mode of Vibrio parahaemolyticus on zebrafish and the immune effects of soybean isoflavones on the Vibrio, two groups of experiments were performed. Ten zebrafish in each group were challenged by intramuscular injecting, surgical injuring and water soaking with different concentration of the Vibrios, respectively. The results show that intramuscular injection could cause death in high concentration of the bacteria. The LC50 is 5.63 × 10^7 CFU·mL^-1 （48 h）. No death was observed in immersion treatment while low mortality occurred in wounding treatments. As a result, intramuscular injection is an important infection mode of Vibrio parahaemolyticus on zebrafish. Moreover, the mortality is much lower at low water temperature （ 16℃ ） than that at high water temperature （28℃） in muscular injection treatment, showing that water temperature and the bacterial concentration are important factors for mortality in the injection group. For the immune effect test of soybean isoflavones, the animals were administered feeds with additive of soybean isoflavones at concentration of 0% （as a control） , 0. 5% , 1% and 2% , respectively for fourteen days. The fishes were subsequently subject to muscular injection of Vibrio at the concentration of 3.6 × 10^9 CFU·mL^-1. The results indicate that 1% soybean isoflavones can resist infection, suggesting that it is good to reduce diseases and to be a new immunostimulants in aquaculture practice.

关 键 词： 副溶血弧菌 斑马鱼 感染模式 大豆异黄酮 免疫

领　　域： [农业科学] [农业科学]