作　　者： ; ;

机构地区： 中国水产科学研究院南海水产研究所

出　　处： 《南方水产》 2006年第5期74-80,共7页

摘　　要： 微卫星 DNA 是分布于基因组的1～6个碱基组成的串联重复序列,或简单序列重复。微卫星 DNA 具有多态性高、数量丰富、共显性遗传和分析简单等特点,应用越来越广泛,已成为最受青睐的分子标记之一。微卫星分子标记的获得首次必须从实验生物中分离。分离微卫星 DNA 位点的方法有多种。文章对几种微卫星 DNA 位点分离技术进行介绍和分析比较,为选择合适的分离方法提供参考。 Microsatellite DNAs, or single sequence repeats （SSRs）, are small array of one to six tandemly repeated bases that spread throughout the genome. Microsatellite DNAs are highly polymorphic, abundant, co-dominantly inheritant, simple in practice and readily transferable. Thus they have been widely used in many fields and have become one of the most popular molecular markers. Yet microsatellite DNAs need to be isolated de novo from species that are being examined for the first time. There are a number of ways to obtain microsatellite markers from genomic DNA. The aim of the present paper is to review the state-of-the-art methods of microsatellite DNA isolation for reference.

关 键 词： 微卫星 分离方法 多态性 磁珠富集

领　　域： [生物学]