作　　者： ; ; ; ; ; (李恒聪）;

机构地区： 深圳市血液中心

出　　处： 《中国实验血液学杂志》 2008年第3期694-698,共5页

摘　　要： 本研究探讨中国最大少数民族壮族Y染色体特异短串联重复序列(Y-STR)单倍型的遗传多态性。采用多重PCR扩增和ABIPrismTM3100基因测序仪荧光检测方法,对85名壮族非亲缘男性个体的9个短片段长度Y-STR基因座,进行基因频率和单倍型频率的调查。结果表明:在85名非亲缘男性个体中,除DYS426基因座多态性较低,其余8个基因座的GD值的分布在0.4387-0.8129之间。9个Y-STR基因座的单倍型共有70种,单倍型多样性达0.9926。结论:壮族非亲缘男性个体Y-STR单倍型的遗传多态性丰富,与我们以往的南方汉族非亲缘男性群体遗传资料相比较,具有显著的差异。 The aim of this study was to investigate the genetic polymorphism of Y-chromosome specific short tandem repeat （Y-STR） loci in Zhuang ethnic group of China. Nine Y-STR loci were amplified by single multiplex and the PCR products were detected by using ABI PrismTM 3100 DNA Sequencer. The allele frequencies and haplotype frequencies at 9 Y-STR loci were determined in a total of 85 unrelated male individuals from Zhuang ethnic group of China. The results indicated that in the 85 unrelated male individuals, except for the DYS426 locus with a low GD value, the GD values for other 8 Y-STR loci ranged from 0. 4387 to 0. 8129. A total of 70 haplotypes at 9 Y-STR loci were found, the haplotype diversity was 0. 9926. It is concluded that the haplotype polymorphism of 9 Y-STR loci are highly polymorphic in Zhuang ethnic group and also significantly different from our previous reported data of unrelated male individnals in southern Chinese Han population.

关 键 词： 染色体 染色体短串联重复序列 单倍型 遗传多态性 群体遗传学

领　　域： [生物学]