作　　者： ; ; ;

机构地区： 长春工业大学化学与生命科学学院

出　　处： 《吉林大学学报（医学版）》 2008年第3期361-363,共3页

摘　　要： 目的：研究小分子化合物8-氧-8H-苊并[1，2-b]吡咯-9-腈的氨基或卤素取代衍生物（S1）对人雄性激素非依赖型前列腺癌PC3细胞的作用及机制。方法：常规培养PC3细胞，分为10．00、5．00、1．00、0．50、0．10、0．05和0．01μmol·L^-1 S1化合物组，同时设培养肿瘤细胞对照组和抗癌药物环磷酰胺（5．00 μmol·L^-1）对照组，采用MTT法检测PC3细胞增殖抑制率，流式细胞术检测诱导PC3细胞凋亡作用，Caspase3、8和9试剂盒检测细胞凋亡途径。结果：0．10～10．00μmol·L^-1 S1化合物组，PC3细胞生长抑制率明显高于环磷酰胺对照组（P〈O．01）；5．00和10．00μmol·L^-1 S1化合物组PC3细胞凋亡率明显高于培养肿瘤细胞对照组（P〈0．01）；10．00μmol·L^-1 S1化合物作用于PC3细胞后不同时间（1、2、4及6h）细胞Caspase 3和Caspase 9活性均显著高于培养肿瘤细胞对照组（P〈0．01），Caspase 8活性未见明显改变。结论：S1化合物能够通过诱导PC3细胞凋亡而导致细胞死亡，其作用机制是线粒体途径，进一步证实S1化合物作为肿瘤治疗药物的可行性。 Objective To study the effect of small heterocyclic compound 8-oxo-3-thiomorpholino-8H-acenaphtho [1, 2-b] pyrrole-9-carbonitrile （S1） on apoptosis of human androgen independent prostatic carcinoma cells line （PC3） and its mechanism. Methods PC3 cells were cultivated, and divided into different groups： 10. 00, 5.00, 1.00, 0. 50, 0. 10, 0.05 and 0. 01 μmol·L^-1 S1 groups, meanwhile, PC3 control group and cyclophosphamide group were set up. MTT was used to detect the inhibitory rate of PC3 cell proliferation. Flow cytometry was used to detect the inducing effect of S1 on apoptosis of PC3 cells. Caspase 3, 8, 9 kits were used to detect apoptosis route. Results The inhibitory rates of PC3 ceils induced by 0. 10--10. 00 μmol·L^-1 S1 were significantly higher than that in cyclophosphamide group （P〈0. 01）. The apoptotic rates induced by 5.00 and 10.00 μmol·L^-1 S1 were significantly higher than that in PC3 control group （P 〈 0. 01）. When PC3 cells were treated with 10.00 μmol·L^-1 S1 for 6 h, the activity of Caspase3 was significantly higher than that in PC3 control group （P〈0.01）. When PC3 cells were treated with 10. 00 μmol·L^-1 S1 for 4-6 h, the activities of Caspase 9 were significantly higher than that in control group （P〈0. 01）, but the activity of Caspase 8 did not change significantly.Conclusion S1 compound can induce apoptosis of PC3 cell line and death, and its mechanism may be related to mitochondria.

关 键 词： 细胞系 细胞凋亡 机制

领　　域： [生物学]