作　　者： ; ; ;

机构地区： 西北农林科技大学园艺学院农业部西北园艺植物种质资源与遗传改良重点开放实验室

出　　处： 《园艺学报》 2008年第5期681-686,共6页

摘　　要： 以大白菜温敏雄性不育系TsCMS7311（A）及其保持系7311（B）的小花蕾（长度大约1．5mm）为材料，活性凝胶电泳分析发现：β-酯酶同工酶与育性转化关系密切，正常不育系比保持系缺少迁移率为0．683、0．718和0．742的3条酶带；随着育性的转化，酶谱接近保持系带型；当育性回复到正常的不育状态时，其同工酶酶谱也随之回复到正常的不育系带型。在低温处理后的不育系TsCMS7311（A^T）育性转化期间，回收与雄性育性转化相关的β-酯酶带区，双向凝胶电泳分析，结果表明，保持系B和转化后的不育系A^T的双向电泳图谱相似，第1向IEF电泳中各获得了2条特异谱带，等电点在7．7附近；第2向SDS电泳中各得到了6种β-酯酶多肽，分子量在55～62kD之间。 This paper studied the change of β-EST isoenzymes of small buds （ 1.5 mm or so） in the thermo-sensitive CMS of Chinese cabbage （ Brassica campestris L. ssp. pekinensis ） TsCMS7311 （ A ） and maintainer line 7311 （B） during the fertility changeover. By one-dimensional GEL electrophoresis analyzing, we found that there were three β-EST isoenzymes related to the sterility changeover closely, their Rf were 0. 683,0. 718 and 0. 742 respectively. During sterile stamen changed into fertile in male sterile line （ AT ） after low temperature treatment, the β-EST zymograms changed into maintainer line＇s （B） too ; While fertile stamen in male sterile line （AT） came back into sterile, the β-EST zymograms came back into the natural male sterile line＇s （A） again. Enriched these β-EST isoenzymes with electroelution, then separated and purified these β-EST isoenzymes with two-dimensional GEL electrophoresis, we gained six polypeptides, whose pI values were about 7.7 and molecular mass were between 55 kD and 62 kD.

关 键 词： 大白菜 育性转化 酯酶同工酶 双向电泳

领　　域： [农业科学] [农业科学]