作　　者： ; ; ;

机构地区： 西北农林科技大学动物科技学院

出　　处： 《畜牧兽医学报》 2008年第5期536-539,共4页

摘　　要： 以北京黑猪为研究对象,选取视黄醇结合蛋白4(Retinol-binding proteins 4,RBP4)基因为繁殖性状的候选基因,其扩增产物经MspⅠ酶切后,在北京黑猪群体中发现AA、AB和BB 3种基因型,其A、B等位基因的频率、多态信息含量、杂合度分别为0.529 6、0.470 4、0.374 1和0.498 2,表明该位点处于中度多态。χ2适合性检验表明北京黑猪群体该位点处于Hardy-Weinberg平衡状态(P>0.05)。用SAS 8.0软件最小二乘法拟合线性模型,将不同基因型与总产仔数(TNB)、产活仔数(NBA)和出生重(WB)进行了关联分析,结果表明:经产母猪各基因型间的TNB、NBA、WB差异均不显著(P>0.05)。初产母猪BB型比AA和AB型个体的TNB分别多1.04(P<0.05)和0.31头;BB和AB型比AA型个体的NBA分别多1.47(P<0.05)和1.35头。基因效应分析结果表明,初产母猪该位点的B等位基因对TNB、NBA和WB都表现为正效应,分别使各性状增加了0.282 0头、0.408 5头和0.000 9kg,即B等位基因可能为北京黑猪繁殖性能的有利等位基因。 RBP4 gene was regarded as a candidate gene for reproduction traits of Beijing Black pig in this study, and the polymorphism was detected by PCR-RFLP with restriction endomuclease Msp I . Three genotypes were found in Beijing Black pig, and the frequencies of allele A and B, polymorphism information content and heterozygosity were 0. 529 6, 0. 470 4, 0. 374 1 and 0. 498 2, respectively, which revealed a moderate polymorphism in this site. Statistical results of 3（2 test indicated that this site fitted Hardy-Weinberg equilibrium （P〉0.05）. Meanwhile, the effect of polymorphism of RBP4 gene on TNB, NBA and WB were analyzed, the results indicated that the TNB, NBA and WB of multiparous sows with either genotype showed no significant difference （P〉0.05）. The sows of first parity with genotype BB had 1.04 and 0.31 litter size of TNB more than those with genotypes AA and AB, and the pigs of first parity with genotypes BB and AB had 1.47 and 1.35 litter size of NBA more than those with genotype AA, respectively. The results of genotype effective analysis indicated that allele B had the positive effect on TNB, NBA and WB, which might increase 0. 282 0, 0. 408 5 and 0. 000 9, respectively, that was to say, allele B might be a favourable allele on TNB, NBA and WB.

关 键 词： 北京黑猪 基因 多态性 繁殖性状 候选基因

领　　域： [农业科学] [农业科学]